阴离子交换色谱纯化甲型肝炎病毒(YN5株)的研究  

Studies on the purification hepatitis A vaccine(YN5 strain)with anion exchange chromatography

作　　者：马波 向左云[2] 钟光禄 陶林 何丽芳 陈尔佳 

机构地区：[1]云南沃森生物技术有限公司,昆明650033 [2]成都军区联勤部军事医学研究所,昆明650032

出　　处：《微生物学免疫学进展》2004年第1期5-8,共4页Progress In Microbiology and Immunology

基　　金：国家高技术研究发展专项经费资助项目( 2 0 0 2AA2Z331 3);云南省科技计划资助项目( 2 0 0 1XY0 1 )

摘　　要：对阴离子交换色谱纯化HAV的合适条件进行了探索。先使用“试管法”研究DEAESepharoseFastFlow凝胶结合HAV及病毒解离的条件 ,然后分别使用线性阶段洗脱和阶段梯度洗脱在柱色谱上进行了HAV的纯化。结果表明经过阴离子交换色谱纯化得到的病毒保持有抗原性和免疫原性 ,HAV抗原回收率大于 85% ,杂蛋白去除率大于 80 % ,纯化的病毒样品中的内毒素与宿主DNA的含量也大大降低 ,证明阴离子交换色谱可用于HAV疫苗的纯化。The production of hepatitis A vaccine,which was carried out on anion exchange chromatography by using DEAE Sepharose FF in this study, is a very effe ctive alternative to gradient centrifugation.The hepatitis A virus(HAV)retains it s antigenicity and immunogenicity very well,and recovery of HAV antigen is well above 85%.On the other hand,the impurities have been removed more than 80%.The content of contaminated endotoxin and host DNA are also greatly reduced in the process.

关 键 词：甲型肝炎病毒 YN5株 阴离子交换色谱 纯化 

分 类 号：R392[医药卫生—免疫学]