S/D灭活血浆内脂包膜病毒及病毒灭活血浆的研究  

Inactivation of enveloped viruses in human plasma by solvent/detergent (S/D)

作　　者：赵宜为[1] 常娅莉[1] 王巍[1] 刘燕[1] 尚立群[1] 梁艳[1] 丁宜清[1] 

机构地区：[1]兰州生物制品研究所,兰州730046

出　　处：《微生物学免疫学进展》2004年第1期17-19,共3页Progress In Microbiology and Immunology

摘　　要：研究磷酸三丁酯 (TNBP) /TritonX 10 0对血浆内脂包膜病毒的灭活效果。用VSV病毒和Sindbis病毒作指示病毒 ,加入血浆后再加磷酸三丁酯 /TritonX 10 0 ,观察病毒的滴度变化及对血浆蛋白的影响。结果发现终浓度各为1%的磷酸三丁酯 /TritonX 10 0在 60min内可以灭活血浆内的两种指示病毒 ,而血浆蛋白的组成和功能变化很小。经层折、超滤后血浆内磷酸三丁酯和TritonX 10 0的残余量分别低于 5μg/ml,表明S/D处理血浆的安全性和治疗作用都很好 ,其制剂冰冻血浆或冻干血浆可用于临床治疗凝血因子缺乏症 。S/D treatment was investigated to inactivate lipid-enveloped viruses,in human plasma. Both vesicular stormatis virus and Sindbis virus were used as indicators for virus inactivation. The treatment did not significantly alter the protein composition and coagulative function of plasma, but lipid-enveloped vi ruses were effectivety inactivated by S/D in combination with 1% tri(n-butyl) phosphate and 1% Triton X -100 incubation at 30℃ within 60 minutes. After completion of processing by octadecy1 resin, the residual S and D were reduced to less than 5μg/ml, respectivel y. It suggests the preparations of freeze or freeze-dried plasma treated by S/D may be used in clinical therapy for deficiency of coagulation factors and/or transfution in hypovolaemia.

关 键 词：磷酸三丁酯/Triton X-100(S/D) 血浆 病毒灭活 

分 类 号：R392[医药卫生—免疫学]