肾性高血压大鼠重要脏器钙调神经磷酸酶活性的变化  

The Alteration of Calcineurin Activity in Vital Tissues and Organs of 1K1C Renal Hypertensive Rats

作　　者：陈雯[1] 谢晓华[1] 常连庆[1] 孙愚[1] 张颖[1] 卢文宁[1] 李朝晖 刘宁 刘秀华[1] 

机构地区：[1]解放军总医院南四科,北京100853 [2]解放军272医院

出　　处：《高血压杂志》2004年第1期66-69,共4页Chinese Journal of Hypertension

基　　金："十五"军队医药卫生科研基金重点课题资助 (0 2Z0 0 1 0 )

摘　　要：目的　观察一肾一夹肾性高血压大鼠重要脏器钙调神经磷酸酶 (CaN)活性的变化。方法　取肾性高血压大鼠及假手术组大鼠不同器官组织 ,以PNPP为底物测定各组织CaN活性 ,应用免疫组织化学方法测定CaN催化亚单位β亚型 (CaNAβ)在大鼠心肌细胞中的分布。结果　肾性高血压大鼠组心脏、脾、肾脏、肺、脑CaN活性较假手术组分别上升 175 %、90 %、84 %、5 7%、4 3% (均P <0 0 5 ) ,肝脏CaN活性较对照组下降 2 7% (P <0 0 5 ) ,主动脉中CaN活性无明显变化。免疫组织化学显示肾性高血压大鼠心肌细胞胞浆中CaNAβ阳性棕色染色颗粒较对照组明显增多。结论　肾性高血压大鼠心、脾、肾脏、肺、脑和肝脏中CaN活性均有显著变化 ,心脏CaN活性增高最著 ,且CaNAβ在心肌细胞胞浆中表达增加 ,并可能在心肌细胞重构中发挥重要作用。Objective To investigate the alteration of calcineurin (CaN) activity in vital tissues and organs in the renal vascular hypertensive rats(RHR). Methods The models of renal vascalar hypertensive rats were established by the operation of “one-kidney one-clip”. Thirteen Wistar rats were divided into two groups randomly: operation groups(RHR,n =7) and sham operation groups(control,n =6). CaN activity was measured in various tissues and organs by using PNPP as substrate 4 weeks after operation. By using immunohistochemical staining methods,the distribution of CaNA β in cardiac myocytes was examined. Results Compared with controls,CaN activity in heart,spleen,kidney,lung,brain of RHRs was increased by 175%,90%,84%,57%,43%,respectively( P <0.05),while in liver was decreased by 27%( P <0.05). In immunohistochemical staining study,there was an increase of postive brown immunostaining of CaNA β in myocardial cytoplasmic of RHRs. Conclusion CaN activity in heart,spleen,kidney,lung,brain,liver underwent remarkable changes in RHRs. The maximum increase of CaN activity was found in heart. Upregulated expression of immunostaining of CaNA β in myocardial cytoplasmic was found in of RHRs,suggesting its role in cardiomyocyte remodeling.

关 键 词：肾性高血压 钙调神经磷酸酶 脏器 活性 

分 类 号：R544.1[医药卫生—心血管疾病]