拟南芥叶细胞游离钙离子的测定  

The Detection of Cytosolic Free Ca2+ in Cells of Arabidopsis thaliana Leaves

作　　者：孙清鹏[1] 王小菁[1] 

机构地区：[1]华南师范大学生命科学学院广东省植物发育生物工程重点实验室,广东广州510631

出　　处：《热带亚热带植物学报》2004年第1期79-82,共4页Journal of Tropical and Subtropical Botany

基　　金：国家自然科学基金(39970079)

摘　　要：低温(4℃)条件下将钙离子荧光探针Fluo-3/AM导入拟南芥叶细胞,利用激光共聚焦显微技术检测了胞内钙离子荧光强度的分布。实验证明,低温导入Fluo-3/AM法测定拟南芥叶细胞中钙离子荧光强度的变化切实可行。茉莉酸(JA)处理能够诱导胞内游离钙离子浓度的升高。Fluorescent probe Fluo-3/AM was loaded into the cells of Arabidopsis th aliana leaves under lowtemperature at 4℃ to measure the fluorescence intensity of intracellular Ca2+ u sing laser scanning confocalmicrotechnique. When the leaves were treated with 100 μmol/L jasmonic acid, cyt osolic free Ca2+ increased within one min and reached a peak after 5 min. The ex periment shows that this is a feasible method to detect intracellular Ca2+ in in tact leaves of A. thaliana.

关 键 词：拟南芥 Fluo-3/AM 钙离子 茉莉酸 

分 类 号：Q942[生物学—植物学]