强骨抗萎方对模拟失重条件下体外培养成骨细胞的影响  

Effects of Qiang Gu Kang Wei compound on osteoblasts cultured in vitro in simulated weightlessness condition

作　　者：李勇枝[1] 石宏志[1] 范全春[1] 陶英[1] 白桂娥[1] 段渊儒[1] 谢俊水[1] 

机构地区：[1]航天医学工程研究所,北京100094

出　　处：《中华航空航天医学杂志》2003年第4期196-199,共4页Chinese Journal of Aerospace Medicine

摘　　要：目的　探讨复方强骨抗萎方对模拟失重条件下体外培养成骨细胞的影响 ,拟揭示该方对抗骨丢失的分子生物学机制。　方法　分离乳鼠颅骨成骨细胞 ,体外培养后 ,分为正常对照、失重模型、强骨抗萎方大剂量 ( 2 .0 % )、中剂量 ( 1.0 % )和小剂量 ( 0 .5 % ) 5组。置回旋器 ,30r/min ,连续回旋 6 0h模拟失重 ,观察该方对回旋 12h、36h和 6 0h时大鼠成骨细胞碱性磷酸酶 (ALP)和骨钙素(BGP)生成及ALP基因转录水平 (mRNA)的影响。　结果　与正常对照组比较 ,模型组成骨细胞在回旋 12h、36h和 6 0h不同时间点的细胞培养液中的ALP活性均明显降低 ,其中 12h和 6 0h时差异显著 (P <0 .0 5 ) ;且ALPmRNA表达低微 ;BGP活性均减低 ,6 0h时差异显著 (P <0 .0 5 )。与模型组比较 ,中药复方不同剂量组于回旋不同时间点细胞培养液中的ALP活性均有不同程度升高 (P <0 .0 5 ) ;ALPmRNA表达活性也较高 ;但BGP活性无明显变化。　结论　该方能改善模拟失重条件下成骨细胞的功能 ,缓解其分化抑制状态 ,促进骨基质的形成和成熟。Objective To investigate effects of Qiang Gu Kang Wei compound on osteoblasts cultured in vitro in simulated weightlessness condition, so as to explore its molecular biological mechanism of resisting bone loss. Methods Osteoblasts from skull of suckling rats were separated, cultured in vitro, and divided into five groups (normal control, rotation model, high dose 2 0%, middle dose 1.0% and low dose 0.5% Qiang Gu Kang Wei compound group. The 4 experimental groups of cultured osteoblasts underwent simulated weightlessness in rotating clinostat at a speed of 30rpm for 60h. The activity of both alkaliphosphatase (ALP) and osteocalcin(BGP) as well as ALP mRNA of osteoblasts after different periods of time (12 h, 36 h, 60 h) of rotation were observed. Results Compared with the control, both ALP and BGP activity of osteoblasts in culture fluid after different time periods(12 h, 36 h, 60 h) of rotation decreased significantly in model group( P <0.05). Accordingly, expression of ALP mRNA in this group was lower than that of the control. As compared with model group, the ALP activity and ALP mRNA expression were higher ( P <0.05) in the 3 groups treated with different doses of compound, while the differcences between BGP level in model group and treatment groups were nonsignificant. Conclusion It is suggested that Qiang Gu Kang Wei compound can improve osteoblasts function under simulated weightlessness, alleviate its differential inhibition state, and promote the formation and maturity of bone stroma.

关 键 词：强骨抗萎方 失重模拟 成骨细胞 基因 

分 类 号：R85[医药卫生—航空、航天与航海医学]