Establishment and Identification of Cytoplasmic Male Sterility in Brassica napus by Intergeneric Somatic Hybridization  

作　　者：HUQiong LIYun-chang MEIDe-sheng FANGXiao-ping LiseNHansen SvenBAndersen 

机构地区：[1]KeyLaboratoryofAgriculturalMinistryforOilCropsImprovement,NationalCenterforOilCropsImprovement,InstituteofOilCropResearch,ChineseAcademyofAgriculturalSciences,Wuhan430068,P.R.China [2]DepartmentofAgriculturalSciences,TheRoyalVeterinaryandAgriculturalUniversity,FrederiksbergC,Copenhagen1871,Denmark

出　　处：《Agricultural Sciences in China》2003年第12期1321-1328,共8页中国农业科学（英文版）

基　　金：This study was supported by Chinese National High Technology 863 Program(2002AA207009);National Natural Science Foundation of China(39570442);Chinese Ministry of Education,Chinese Ministry of Personnel,Wuhan Municipal Science and Technology Commission(T20005102019);a Sino-Danish Government Scholarship.

摘　　要：Exploitation of novel cytoplasmic male sterility (CMS) is a main approach for widening the cytoplasmic genetic background of hybrid oilseed rape and avoiding epidemic risk in oilseed rape production. In this study, symmetric somatic hybrids between Brassica napus var. Zhongshuang4 and Sinapis arvensis (Ye- you18) were produced by protoplast fusion. Two of the six established hybrids were male sterile showing trace or no pollen release upon flowering with non- or slightly extended stamens. Using Zhongshuang4 as a recurrent parent to pollinate the male sterile plants, the ratio of male sterile plants increased with the number of backcrosses. As early as in BC 3 generation, most of the sterile families had nearly 100% sterile plants. Up to BC 4 generation, the male sterility became stable and no fertility segregation was observed. All F 1 progenies from tested crosses using restorer and maintainer lines of Polima CMS were 100% sterile, indicating that the established CMS by somatic hybridization is different from Polima CMS. The origin of the cytoplasm and potential use of this novel CMS in oilseed rape breeding were discussed.Exploitation of novel cytoplasmic male sterility (CMS) is a main approach for widening the cytoplasmic genetic background of hybrid oilseed rape and avoiding epidemic risk in oilseed rape production. In this study, symmetric somatic hybrids between Brassica napus var. Zhongshuang4 and Sinapis arvensis (Ye- you18) were produced by protoplast fusion. Two of the six established hybrids were male sterile showing trace or no pollen release upon flowering with non- or slightly extended stamens. Using Zhongshuang4 as a recurrent parent to pollinate the male sterile plants, the ratio of male sterile plants increased with the number of backcrosses. As early as in BC 3 generation, most of the sterile families had nearly 100% sterile plants. Up to BC 4 generation, the male sterility became stable and no fertility segregation was observed. All F 1 progenies from tested crosses using restorer and maintainer lines of Polima CMS were 100% sterile, indicating that the established CMS by somatic hybridization is different from Polima CMS. The origin of the cytoplasm and potential use of this novel CMS in oilseed rape breeding were discussed.

关 键 词：Oilseed rape Protoplast fusion Cytoplasmic male sterility Sinapis arvensis 

分 类 号：S565.403.5[农业科学—作物学]