Extraction DNA from Activated Sludge-Comparing with Soil Sample  

作　　者：谢冰 奚旦立 陈季华 

机构地区：[1]School of Environmental Science and Engineering, Donghua University, Shanghai, 200051

出　　处：《Journal of Donghua University(English Edition)》2003年第1期127-130,共4页东华大学学报（英文版）

摘　　要：DNA directly extraction from activated sludge and soil sample with enzyme lyses methods was investigated in this paper. DNA yield from activated sludge was 3.0 mg/g. MLSS, and 28.2 -43.8 μg/g soil respectively. The resulting DNA is suitable for PCR. By studied methods, higher quality and quantity of sludge DNA could be obtained rapidly and inexpensively from large number of samples, and the PCR product obtained from this protocol was not affected by contaminated higher concentration of heavy metals.DNA directly extraction from activated sludge and soil sample with enzyme lyses methods was investigated in this paper. DNA yield from activated sludge was 3.0 mg/g. VLSS, and 28.2-43.8 μg/g soil respectively. The resulting DNA is suitable for PCR.By studied methods, higher quality and quantity of sludge DNA could be obtained rapidly and inexpensively from large number of samples, and the PCR product obtained from this protocol was not affected by contaminated higher concentration of heavy metals.

关 键 词：DNA extraction PCR (polymerase chain reaction) activated sludge SOIL heavy metal. 

分 类 号：X703[环境科学与工程—环境工程]