山茱萸免疫活性部位F-1C的免疫抑制作用  

Pharmacological Study on Immunosuppressive Fractions F-1C from Fructus corni

作　　者：吕晓东[1] 杨胜1 齐春会[1] 张永祥[1] 茹祥斌[1] 周文霞[1] 吴禾[2] 赵毅民[2] 

机构地区：[1]军事医学科学院毒物药物研究所中药和神经免疫药理研究室 [2]军事医学科学院毒物药物研究所天然药物化学研究室,北京100850

出　　处：《中国天然药物》2004年第1期50-54,共5页

基　　金：国家重点基础研究发展规划项目资助课题(No .G19990 5 44 0 1)

摘　　要：目的 :研究山茱萸免疫活性部位F 1C对小鼠免疫反应的抑制作用。方法 :运用淋巴细胞增殖反应、混合淋巴细胞反应、溶血空斑实验和流式细胞技术观察F 1C体外应用和口服对正常小鼠免疫反应和脾脏T细胞亚群的影响。结果 :F 1C在 10 2 0 0 μg/ml剂量范围内无明显细胞毒性 ,但对小鼠ConA诱导的脾细胞增殖反应、混合淋巴细胞反应和脾细胞抗体生成反应具有明显的抑制作用 ,对LPS诱导的裸鼠脾细胞增殖反应呈剂量依赖性的促进作用。连续 7天口服给予 4 0和 80mg/kg ,明显抑制脾细胞抗体生成反应和脾细胞自发增殖反应 ,对脾脏T细胞亚群CD4+ 细胞数目无明显影响 ,但明显升高CD8+ 细胞百分数 ,使CD4+ /CD8+ 比值降低。同时给药组小鼠T抑制细胞 (Ts)的抑制功能增强。结论 :F 1C对正常小鼠免疫反应具有明显的抑制作用 ,增加Ts细胞数量并增强Ts细胞抑制功能可能是其发挥免疫抑制作用的途径之一。AIM:To investigate the immunosuppressive effect of F 1C,a fraction from the \%Cornus officinals\%, which is one component of Liu Wei Di Huang (LW) decoction. METHOD: The effects of F 1C on immune response were evaluated \%in vitro\% and \%in vivo\%. RESULT: F 1C showed strong inhibiting effect on antibody production response and proliferation of lymphocyte, during concentration range without cytotoxinic effect in vitro. Oral administration of F 1C also significantly inhibited antibody production response and splenocyte proliferation response. F 1C showed no influence on the number of CD 4 + splenocyte, however, it increased the number of CD 8 + splenocyte and decreased the ratio of CD 4 + /CD 8 + , suggesting that F 1C increased the quantity of Ts cell. The inhibition function of Ts was also enhanced by F 1C. CONCLUSION: F 1C possesses potent immunosuppressive effect. Enhancing the activity and quantity of Ts cell is one possible underlying mechanism.

关 键 词：山茱萸 活性部位 F-1C 免疫抑制 中药 

分 类 号：R285.5[医药卫生—中药学]