胎儿脏器亚细胞组分中谷胱甘肽S-转移酶活性分布  

Subcellular Distribution of Glutathione S-transferases in Fetal Organs

作　　者：柳明洙[1] 徐明旭[1] 金海玲[1] 李天洙 尹宗柱[1] 

机构地区：[1]延边医学院生物化学教研室

出　　处：《延边医学院学报》1992年第1期1-4,15,共5页Journal of Medical Science Yanbian University

摘　　要：用差速离心法对胎龄4～7个月水囊引产胎儿的肝、肺、肾、心脏、脾、脑和胃组织的胞浆、线粒体和微粒体进行了分离,并以1.2-二氯-4-硝基苯(CDNB)为底物,测定了上述脏器亚细胞组分中谷胱甘肤S-转移酶(glutathione S-transferase,GST)的活性。结果显示,在上述脏器亚细胞组分中,GST活性主要分布在胞浆,在微粒体和线粒体中亦有分布。以每克湿组织所含GST活性大小顺序排列上述各脏器,依次为脾、心、脑、胃和肝、肾、肺。Subcellular fractions were isolated from fetal liver, lung, kidney, heart, spleen, brain and stomach at 4 to 7 months of age, and glutathione S-transferases(GST) were determined by using 1-chloro-2,4-dinitrobenzene(CDNB) as substrate. GST activities were found mainly in cytosol fraction, and little lower activities of GST were found in mitochondrial and microsomal fractions in all of the organs. According to the GST activities of each organ(U/g wet tissue), it can be put in order as follows: spleen, heart, brain, stomach, liver, kidney, lung.

关 键 词：谷胱甘肽 转移酶 胞浆 胎儿 

分 类 号：Q593.9[生物学—生物化学]