江苏地区汉族人群八个短串联重复序列基因座遗传多态性研究  

Genetic polymorphism at eight STR loci for the Han population in Jiangsu province of China

作　　者：易龙[1] 陈仕林[2] 许争峰[3] 潘永久 杨驰[1] 应锋[1] 丁卫东[1] 王勇[1] 凌立君[1] 

机构地区：[1]南京大学医学院DNA指纹实验室,江苏南京210093 [2]南京军区南京总医院心胸外科,江苏南京210002 [3]南京市鼓楼医院妇产科遗传室,江苏南京210008 [4]江苏高级人民法院司法鉴定处,江苏南京210024

出　　处：《医学研究生学报》2004年第2期117-119,共3页Journal of Medical Postgraduates

摘　　要：目的 :了解江苏地区汉族人群D14S30 6、D1S 818、D3S1338、LPL、F13B、F13A0 1、FESFPS和vWA等八个短串联重复序列 (STR)基因座的遗传多态性 ,并获得该群体的遗传学数据。　方法 :采用Chelex 10 0法从江苏地区无亲缘关系的汉族个体血标本 12 2份中提取DNA。特异引物聚合酶链反应。产物经 6 %变性聚丙烯酰胺凝胶电泳分离 ,银染检测。　结果 :这八个STR基因座在江苏地区汉族人群均具有遗传多态性 ,并符合Hardy Wein berg定律 ,它们的个人识别率范围从F13B的 0 .6 86 9到vWA的 0 .92 85 ,非父排除率范围从F13B的 0 .2 4 99到vWA的 0 .6 0 2 6不等。　结论Objective:To understand the genetic polymorphism at STR loci D14S306, D1S818, D3S1338, LPL, F13B, F13A01, FESFPS and vWA for the Han population in Jiangsu province of China and construct a preliminary database. Methods: Allele and genotype frequencies for six terameric short tandem repeat (STR) loci were established from 122 unrelated Chinese Han individuals in Jiangsu province of China using locus-specific prime polymerase chain reactions (PCR), followed by electrophoresis of the PCR products in denaturing polyacrylamide gels and subsequent detection by silver staining. Results: All loci exhibit a high degree of polymorphism in the Han population in Jiangsu province of China and met Hardy-Weinberg expectations. The discrimination power (DP) ranged from 0.686 9 for F13B to 0.928 5 for vWA and the power of exclusion (PE) ranged from 0.249 9 for F13B to 0.602 6 for vWA. Conclusion: The obtain date can be highly suitable for forensic individualization and paternity tests to Han population in Jiangsu province of China.

关 键 词：聚合酶链反应 遗传多态性 短串联重复序列 江苏地区汉族群体 

分 类 号：R392.2[医药卫生—免疫学]