幽门螺杆菌对胆汁的耐受性研究  

Study of tolerance of Helicobacter pylori to bile

作　　者：黄燕[1] 邹益友[2] 范学工[1] 田雪飞[1] 张艳[1] 

机构地区：[1]中南大学湘雅医院传染科,湖南长沙410008 [2]中南大学湘雅医院消化科,湖南长沙410008

出　　处：《中国现代医学杂志》2004年第3期100-102,共3页China Journal of Modern Medicine

基　　金：国家自然科学基金(30271171);教育部优秀骨干教师基金(教技司2000-65);湖南省自然科学基金(01jjy2114);湖南省卫生厅科研基金(00022)资助

摘　　要：目的　研究H .pylori对胆汁的耐受情况。 方法　将H .pylori与不同浓度的胆汁 (0 .0 1%～1.8% )共同培养 ,观察H .pylori的生长及形态学的变化。 结果　 <0 .1%的胆汁对H .pylori无影响 ,0 .1%～1.2 %的胆汁部分抑制H .pylori的生长 ,≥ 1.4 %的胆汁完全抑制H .pylori的生长。H .pylori在胆汁培养基中可出现球形体和丝状体 ,但耐受胆汁后形态可恢复正常。结论　较高浓度的胆汁可以抑制H .pylori的生长 ,但H .pylori经诱导可耐受胆汁。Objective: To test the tolerance of H. pylori to bile. Methods: H. pylori was cultured on blood agar plates containing human bile at different concentration from 0.01% to 1.8%.Results:Growth of H. pylori was not affected by <0.1% bile, reduced by 0.1%～1.2% bile and inhibited by ≥1.4% bile. Giemsa staining showed the morphology of H. pylori changed from curved bacillus to spheroid and filar. But it can revert to normal form after it is tolerated in bile. Conclusions: Bile inhibits the growth of H. pylori in a dose-effect manner, but H. pylori can tolerate bile after inducement.

关 键 词：幽门螺杆菌 胆汁 耐受性 

分 类 号：R446.5[医药卫生—诊断学]