桃红四物汤干预骨痂微血管新生及VEGF表达的实验研究  

Intervening Effects of Taohongsiwu Decoction on Callus Micrangium Regeneration and Expression of VEGF

作　　者：熊辉[1] 左亚杰[2] 丁志高[1] 杨腊平[2] 何欣[1] 谭新华[2] 

机构地区：[1]湖南中医学院中西医结合系,湖南长沙410007 [2]湖南中医学院附属一医院

出　　处：《中国医师杂志》2004年第2期195-197,共3页Journal of Chinese Physician

基　　金：湖南省自然科学基金项目 (0 1jjy2 0 38)

摘　　要：目的　研究桃红四物汤对骨折断端骨痂组织内微血管新生重建及VEGF表达的影响。方法　利用日本大耳白兔 65只随机分成四组 :三组作右前肢桡骨中段手术骨折造模并分别给予桃红四物汤、炎迪宁片、蒸馏水灌胃 ,另一组为正常对照组 ,在骨折后 7、15、2 1、3 5d ,采用免疫组化方法检测骨痂组织中VEGF表达 ,组织学染色检测骨痂组织内微血管变化 ,并作计数对比分析。结果　桃红四物汤干预组VEGF表达量高于其它各组 ,并在第 15d表达峰值后下降不显著 ,同时 ,各时相点骨痂微血管增殖和塑形优于其他各组。结论　桃红四物汤通过提高骨折后骨痂内VEGF的表达 ,加速断端微血管新生重建 。Objective To study the effect of Taohongsiwu decoction(THSWD) on the VEGF expression and the micrangium regeneration of callus tissue in bone stump. Methods 65 Japanese rabbits were divided into four groups randomly, three groups were given orally with THSWD, Yandining tablet, distilled water respectively after the right radial bone fracture model was established by operation, and the other group served as control. At the 7th,15th,21th and 35th day after fracture, immunohistochemical method was used to detect the expression of VEGF, and the histochemical staining was used to detect the micrangium change of callus tissue in bone stump. Then the data were compared and analysed. Results VEGF expression level was higher in THSWD intvervening group than that in the other groups, and the decrease of VEGF expression was not obvious after the peak value at 15th day. The callus micrangium regeneration and the remodeling of callus in THSWD intervening group were better in all phase than those in the other groups. Conclusion THSWD could improve the VEGF expression of callus tissue, accelerate the micrangium regeneration in bone stump and promote the healing of fracture.

关 键 词：桃红四物汤 骨痂 微血管新生 VEGF 免疫组织化学 骨折愈合 

分 类 号：R285[医药卫生—中药学]