黄芪对单疱病毒性角膜炎患者Th1/Th2细胞因子的调节作用  

Modulatory Effect of Astragalus Membranaceus on Th1/Th2 Cytokine in Patients with Herpes Simplex Keratitis MAO

作　　者：茅苏萍[1] 程凯灵[2] 周韵芬[3] 

机构地区：[1]重庆医科大学应用技术学院附属医院眼科,重庆400050 [2]重庆医科大学附属第一医院中心实验室 [3]重庆医科大学附属第二医院眼科

出　　处：《中国中西医结合杂志》2004年第2期121-123,共3页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：目的:探讨黄芪对单疱病毒性角膜炎(herpes simplex keratits,HSK)患者血清Th1/Th2细胞因子水平的影响。方法:选择106例HSK患者随机分为黄芪治疗组和病毒唑治疗组,治疗前后分别采用双抗体夹心法酶联免疫吸附实验(ELISA)检测外周血白细胞介素-2(IL-2),白细胞介素-4(IL-4),白细胞介素-10(IL-10),γ-干扰素(γ-IFN)水平;并以同样方法检测62名体检正常献血员作健康对照组。结果:HSK患者血清中IL-4、IL-10水平较健康对照组显著增高(P<0.01);IL-2;γ-IFN水平较健康对照组显著降低(P<0.01)。黄芪治疗组患者治疗后,血清IL-4、IL-10水平显著降低(P<0.01),而IL-2、γ-IFN水平显著增加(P<0.01)。病毒唑治疗组治疗前后上述指标无明显改变(P>0.05)。结论:黄芪能调节HSK患者体内Th1/Th2细胞因子的失衡状态,改善患者机体免疫功能紊乱状况,这对HSK患者治疗有重要意义。Objective: To explore the influence of Astragalus membranaceus (AM) on serum cytokines, Th1, including interleukin-2 (IL-2) and γ-interferon (γ-IFN) , and Th2, including interleukin-4 (IL-4) and interleukin-10 (IL-10), in patients with herpes simplex keratitis (HSK). Methods: One hundred and six HSK patients were randomly divided into the AM treated group and the ribavirin treated group. Levels of serum IL-2, IL-4, IL-10 and γ-IFN of all the patients and 62 healthy person, selected from donors for control group, were determined by sandwich enzyme-linked immunosorbent assay (ELISA) technique. Results: Levels of serum IL-4 and IL-10 in HSK patients were significantly higher and those of IL-2 and γ-IFN were significantly lower than those in the healthy control (all P<0.01). These parameters were significantly improved in the patients of the AM group after treatment, but with no change in patients of the ribavirin group. Conclusion: AM can modulate the imbalance state of Th1/Th2 in HSK patients, improve their immune function disturbance, that shows important significance in treating HSK.

关 键 词：黄芪 单疱病毒性角膜炎 TH1/TH2 细胞因子 调节作用 

分 类 号：R276.7[医药卫生—中医五官科学]