人参二醇、三醇皂苷对人骨髓造血祖细胞增殖作用的研究  

Effect of Panaxadiol Saponin and Panaxtrol Saponin on Proliferation of Human Bone Marrow Hemopoietic Progenitor Cells

作　　者：牛泱平[1] 钱煦岱[2] 王文喜[1] 陈小红[2] 高瑞兰[2] 金锦梅[2] 

机构地区：[1]浙江工业大学药学院,杭州310014 [2]浙江省中医院血液病研究所

出　　处：《中国中西医结合杂志》2004年第2期127-129,132,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：中国和澳大利亚国际合作项目;受澳方政府资助(教外司美1996 973);浙江省中医管理局课题(No.972025);浙江省自然基金课题(No.ZD0007)

摘　　要：目的:探讨人参二醇皂苷(panaxadiol saponin,PDS)和三醇皂苷(panaxtrol saponin,PTS)对骨髓造血祖细胞增殖的作用。方法:从人参总皂苷进一步分离纯化出PDS和PTS,用体外造血祖细胞集落形成技术,观察PDS、PTS对小鼠骨髓红系祖细胞(CFU-E、BFU-E)、粒-单系祖细胞(CFU-GM)和多向祖细胞(CFU-Mix)的刺激增殖作用。结果:不同浓度的PDS(2.5～200μg/ml)可促进造血祖细胞增殖;CFU-E,BFU-E,CFU-GM和CFU-Mix集落产率均显著高于对照组(P<0.05),提高率(％)分别达54.9±6.3、48.8±5.1、27.6±4.2和48.9±3.9。而PTS在同样浓度时,CFU-E、BFU-E产率均显著低于对照组(P<0.05);终浓度为200μg/ml时,CFU-E、BFU-E集落产率为零。在CFU-GM培养中,12.5μg/ml浓度的PTS使集落产率提高(29.7±2.2)％(P<0.05);而100μg/ml和200μg/ml的PTS却出现对CFU-GM的抑制作用,抑制率分别为(48.6±3.9)％和100％。结论:PDS是总皂苷内促进造血的有效成分,而PTS对红系祖细胞具有抑制作用,对粒-单系祖细胞的作用呈剂量依赖关系。Objective: To observe the effect of panaxadiol saponin (PDS) and panaxtrol saponin (PTS) on proliferation of human bone marrow hemopoietic progenitor cells (HPC). Methods: PDS and PTS were separated and purified from ginsenosides, and the effects on HPC were studied using in vitro hemopoietic progenitor cell colony-forming technique, by observing the proliferation of human burst forming unit-erythroid progenitor (BFU-E), colony-forming unit-erythroid (CFU-E), colony-forming unit-granulocyte/macrophage (CFU-GM) and colony-forming unit-pluripotent hemopoietic progenitor (CFU-Mix) in mice after PDS and PTS stimulation. Results: Different concentration of PDS (2.5-200μg/ml) could stimulate the proliferation of HPC obviously, showing increase of CFU-E, BFU-E, CFU-GM and CFU-Mix by 54.9±6.3% , 48.8±5.1% , 27.6±4.2 % and 48.9±3.9% respectively, which was higher than that of the control group. While stimulated by PTS of the same concentration, the CFU-E and BFU-E was lower than that of control significantly (P<0.05); when the terminal concentration of PTS was 200 μg/ml, CFU-E and BFU-E was zero respectively. In the CFU-GM culture, PTS in concentration of 12.5μg/ml could cause the proliferation increased by 29.7±2.2% (P<0.05), but in concentration of 100μg/ml and 200μg/ml, it showed inhibitory effect on CFU-GM, the inhibition rate being 48. 6±3.9% and 100% respectively. Conclusion: PDS is the effective component of ginsenosides in stimulating proliferation of human bone marrow HPC. PTS is an component with inhibitory action on proliferation of CFU-E and BFU-E and its effect on CFU-GM was depending on its concentration.

关 键 词：人参二醇皂苷 骨髓造血祖细胞 增殖作用 人参三醇皂苷 集落 

分 类 号：R285[医药卫生—中药学]