几种作用因子对多年生黑麦草组织培养影响的研究  

Factors Effecting on Tissue Culture of Perennial Ryegrass ( Lolium perenne L.)

作　　者：刘文真[1] 玄松南[2] 陈惠哲[2] 朱睦元[1] 孙宗修[2] 

机构地区：[1]浙江大学生命科学学院,浙江杭州310012 [2]中国水稻研究所农业部水稻生物学重点实验室,浙江杭州310006

出　　处：《林业科学研究》2004年第1期95-101,共7页Forest Research

基　　金：2001-2003年浙江省科学技术厅项目"应用转基因技术培育草坪草新品种及产业化研究"(NO.011102188);2003-2005年国家自然科学基金项目"应用抗冷冻基因延长草坪草绿色期的研究"(NO30270942)

摘　　要：以多年生黑麦草成熟种子为外植体进行了愈伤组织诱导和分化的研究。结果表明:dicamba替代2,4 D,蔗糖替代麦芽糖可以显著提高愈伤组织诱导率和植株再生率;在一定的浓度范围内(3～9mg·L-12,4 D)2,4 D浓度的升高可明显提高愈伤组织的诱导率,但同时却降低了分化率;在愈伤诱导培养基中同时使用两种生长激素(2,4 D和NAA)的效果要好于单独使用一种生长激素(2,4 D)的效果;水解酪蛋白、脯氨酸和谷氨酰胺浓度的增加并没有促进植株再生率的升高。Plantlets were regenerated from seed-derived callus of perennial ryegrass ( Lolium perenne ). The replacements of 2,4-D by dicamba, maltose by sucrose in the callus induction medium significantly improved callus induction frequency and overall plant regeneration ability. From 3 mg·L^(-1) to 9 mg·L^(-1), the stepwise increase of the 2,4-D concentration in maltose containing callus induction medium obviously increased callus induction frequency but decreased callus differentiation frequency. The callus induction medium supplemented with two auxins (2,4-D and NAA) was much better than that with 2,4-D individual. Elevating concentrations of casein hydrolysate, proline and glutamine did not improve overall plant regeneration frequency.

关 键 词：草坪草 多年生黑麦草 组织培养 愈伤组织 外植体 碳源 生长激素 培养基 

分 类 号：S688.4[农业科学—观赏园艺] S543.6[农业科学—园艺学]