纵坑切梢小蠹伴生菌云南半帚孢毒素活性的生物检测  

Bioassay of the Pathogenic Toxin of Leptographium yunnanense , A Fungus Associated with Tomicus piniperda

作　　者：廖周瑜[1] 叶辉[1] 

机构地区：[1]云南大学生命科学学院,云南昆明650091

出　　处：《林业科学研究》2004年第1期102-105,共4页Forest Research

基　　金：云南省应用基础研究基金项目(1999C0012M)资助

摘　　要：比较研究了不同生物检测材料及方法在纵坑切梢小蠹伴生菌———云南半帚孢毒素的生物活性检测上的适应性,结果表明该毒素不仅对云南松产生病害作用,对思茅松和华山松同样产生病害作用。用切根幼苗为材料对该毒素进行生测,所需时间虽较短,但幼苗所产生的症状较复杂,不易观察和确定;以松树及其木段为材料将该毒素进行接种试验,则在韧皮部位形成明显的反应区,与接菌产生的反应症状一样,容易确定,但木段上出现反应症状的时间较短,因此,以木段为材料,采用接种法进行该毒素活性检测较好。Pathogenic toxin of Leptographium yunnanense associated with Tomicus piniperda could pathogeny not only to Pinus yunnanensis ,but also to P.armandii and P.kesiya var. langbianensis . The symptoms were very complicated and not easy to be observed and determined when bioassaying the toxin with seedlings cutted roots, however, when bioassaying the toxin with sampling trees and its wood blocks, the symptoms would be observed clearly, and as the same as they were induced by inoculating the fungus to the host trees. The bioassay results would be observed sooner (about 5 days) when bioassaying the toxin with wood block, so it was better to inoculate the pathogenic toxin to the wood blocks for bioassay.

关 键 词：纵坑切梢小蠹 伴生菌 云南半帚孢 毒素 生物活性检测 

分 类 号：S763.712.4[农业科学—森林保护学] S763.38[农业科学—林学]