不同来源的硫酸乙酰肝素的制备及理化性质研究  

Study on preparation and properties of heparan sulfates from different sources

作　　者：姬胜利[1] 张琳[2] 迟延青[3] 崔慧斐[2] 曹吉超[2] 管华诗[1] 

机构地区：[1]中国海洋大学海洋药物与食品研究所,山东青岛266003 [2]山东大学生化与生物技术药物研究所,山东济南250012 [3]山东省立医院药剂科,山东济南250021

出　　处：《中国生化药物杂志》2004年第1期13-16,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家自然基金资助项目 ( 3 0 3 70 613 )

摘　　要：目的从不同来源制备硫酸乙酰肝素 (HS) ,并测定其理化性质。方法利用酶解法提取分离糖胺聚糖混合物 ,采用乙醇分级沉淀和离子交换柱色谱、凝胶过滤柱色谱等方法将HS纯化 ,醋酸纤维素薄膜电泳 (CAME)测定其纯度。结果所得各种来源的HS在CAME电泳显示一条带 ,且理化性质、生物活性和红外光谱各不相同。PurposeTo prepare heparan sulfate(HS) from different sources, and to determine their physico-chemical properties.MethodsGlycosaminoglycans were extracted and separated from different sources with enzymolysis proteins, then the HS was purified by fractional precipitation with ethanol, ion-exchange chromatography and gel filtration chromatography. The purity of HS was determined by cellulose acetate membrane electrophoresis (CAME).ResultsAll the HS products from different sources had a single zone in CAME. They vary in physico-chemical properties, bioactivities and IR spectra.ConclusionHS from different sources vary in structure and properties.

关 键 词：硫酸乙酰肝素 纯化 测定 

分 类 号：Q538.03[生物学—生物化学]