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作 者:刘颖华[1] 何开泽[1] 杨敏[1] 蒲蔷[1] 张军峰[1]
出 处:《应用与环境生物学报》2004年第1期46-50,共5页Chinese Journal of Applied and Environmental Biology
基 金:中国科学院知识创新工程项目;中国科学院成都地奥科学基金(DASF)资助项目~~
摘 要:从传统的川产道地中药材川牛膝中提取多糖RCP ,用氯磺酸 -吡啶法制备了它的硫酸酯衍生物S RCP .产物经红外鉴定 ,证实RCP硫酸酯化后羟基部分被硫酸基取代 ,在 12 5 0cm-1和 810cm-1处显示了硫酸基的特征吸收峰值 .w(S) =8.2 7% ,取代度Ds=0 .5 6 9.体外实验研究了RCP和S RCP对非洲绿猴肾细胞 (Verocellline)的细胞毒性和抗单纯疱疹病毒 2型 (HSV 2 )的活性 .细胞病变效应法 (CPE)和MTT法结果表明RCP在 6 .0mg/mL以下 ,S RCP在 4 .0mg/mL以下均无细胞毒性 ,RCP本身无抗病毒活性 ,经硫酸酯衍生后的S RCP获得抗病毒活性 ,0 .2 5~ 4 .0mg/mL的S RCP能很好地抑制HSV 2引起的细胞病变 .以 0 .5mg/mL的无环鸟苷 (ACV)作为阳性对照 ,结果显示当S RCP浓度为2 .0mg/mL和 4 .0mg/mL时有显著的抗病毒作用 ,效果与阳性ACV相当 .图 1表 8参 2The sulfated derivative of RCP, a kind of polysaccharide extracted from Cyathula officinalis Kuan was prepared by chlorosulfur acid-pyridine method. In IR analysis, the resulting derivative (S-RCP) showed the characteristic adsorption of sulfo-group at 1 250 cm -1 and 810 cm -1, indicating the hydroxy groups of RCP, was partially esterified by chlorosulfur acid. The sulfur content of S-RCP was 8.27% and the average degree of substitution D s was 0.569. Cytotoxicity and antiviral activity of both RCP and S-RCP were studied in Vero-cell culture system. RCP with concentrations below 6.0 mg/mL and S-RCP below 4.0 mg/mL showed no cytotoxicity to Vero cell. S-RCP could inhibit the cytopathic effect caused by herpes simplex virus type 2 (HSV-2) at concentrations of 0.25~4.0 mg/mL, while RCP itself showed no antiviral activity in the same condition. Further studies proved that S-RCP with concentrations of 2.0 mg/mL and 4.0 mg/mL expressed equal antiviral activity with the commonly used antiviral drug ACV of 0.5 mg/mL. Fig 1, Tab 8, Ref 20
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