贯叶连翘提取物和盐酸赖氨酸抗疱疹病毒复方筛选  

Antiviral activity of complex of Hypericum perforatum Lextract and lysine hydrochloride on herpes virus

作　　者：胡然[1] 库宝善[1] 张永鹤[1] 贾永蕊[1] 姚海燕[1] 段淑敏[2] 

机构地区：[1]北京大学医学部药理学系,北京100083 [2]中国预防医学科学院病毒学研究所诊断二室,北京100052

出　　处：《中国临床药理学与治疗学》2004年第2期136-139,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的 :评价贯叶连翘提取物和盐酸赖氨酸复方抗疱疹病毒活性 ,有效复方在整体动物的安全性。方法 :(1 )采用细胞病变效应法 (CPE) ,测定贯叶连翘提取物与盐酸赖氨酸 3种复方 (分别以质量比 1∶1、1∶ 2和 2∶ 1配比 )对Vero细胞和 2BS细胞的细胞毒性 ,以及对单纯疱疹病毒I型 (HSV 1 )和水痘 带状疱疹病毒 (VZV)的抗病毒活性。 (2 )一次灌胃(ig)给予小鼠复方 (2∶ 1 ) ,观察 1 4d ,测定LD5 0 。结果 :贯叶连翘提取物和盐酸赖氨酸 3种复方体外对HSV 1和VZV表现不同程度的抗病毒活性 ,最小有效浓度(MIC)和半数有效浓度 (IC5 0 )范围分别为 9.7～1 9.5mg·L- 1和 6 .8～ 9.7mg·L- 1,测得复方 (2∶1 )单次小鼠ig的LD5 0 为 1 1 .7g·kg- 1,95 %置信区间为 6 .5～ 1 7.2g·kg- 1。结论 :贯叶连翘提取物和盐酸赖氨酸复方体外具有较好抗HSV I和VZV作用 ,2∶AIM: To study the antiviral effects of the complex of H ypericum perforatum L and lysine hydrochloride against herpes simplex v irus type 1 and varicella-zoster virus (VZV), and to evaluate the LD 50 of the complex in mice. METHODS: The cytotoxities to Vero cells an d 2BS cells and the antiviral activities were evaluated by cytopathic effect (CP E) inhibition assay. ICR mice were administrated(ig)the complex (21) to dete rmine LD 50 . RESULTS: The complex significantly inhibited t he CPE of HSV-1 and VZV. MIC ranged from 9.7 to 19.5 mg·L -1 , IC 50 ranged from 6.8 to 9.7 mg·L -1 . LD 50 of the complex with ratio 21 was 11.7 g·kg -1 with 95% confidence inter val of 6.5 - 17.2 g·kg -1 in ICR mice. CONCLUSION: The complex of Hypericum perforatum L and lysine hydrochlor ide has good effects on inhibiting HSV-I and VZV in vitro. The complex (21) is safe in mice.

关 键 词：贯叶连翘提取物 盐酸赖氨酸 复方 单纯疱疹病毒 水痘-带状疱疹病毒 LD50 

分 类 号：R977.4[医药卫生—药品] R978.7[医药卫生—药学]