硒和/或维生素E对实验性高脂血症大鼠心、肝、肾及血清脂质过氧化和一氧化氮含量的影响  

Effects of selenium and Vitamin E in combination and separateness on lipid peroxidation and NO contents of hearts, livers, kidneys and serum in rats with hyperlipidemia

作　　者：周春阳[1] 冉利[2] 张翔[1] 徐彬[1] 蔡春燕[1] 范萍[1] 

机构地区：[1]川北医学院药物研究所,药理教研室,四川南充637007 [2]川北医学院药物研究所,化学教研室,四川南充637007

出　　处：《中国临床药理学与治疗学》2004年第2期212-215,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：四川省教育厅重点科研项目 (№ :2 0 0 0 A33)

摘　　要：目的 :观察硒和 或维生素E对高脂饲料所致实验性高脂血症大鼠心、肝、肾及血清脂质过氧化和一氧化氮含量的影响 ,探讨硒和维生素E抗脂质过氧化的作用。方法 :采用高脂饲料致大鼠实验性高脂血症 ,然后分组给予经预试后的硒和 或维生素E ,4wk后行乙醚麻醉取血 ,同时取心、肝、肾组织作匀浆 ,采用硫代巴比妥 (TBA)法测各组织或血清丙二醛 (MDA)含量以反映脂质过氧化水平 ;采用硝酸还原酶法测定上述组织的一氧化氮 (NO)含量。结果 :高脂饲料可致实验性大鼠高脂血症 ,与对照组比较 ,甘油三脂 (TG)、极低密度脂蛋白胆固醇 (VLDL C)升高 (P <0 .0 5) ;总胆固醇 (T CHOL)、低密度脂蛋白胆固醇 (LDL C)升高 (P <0 .0 1 )。高脂饲料可致部分组织或血清的脂质过氧化作用加强 ,增加各实验组织MDA含量 ,其中肝脏和血清MDA较对照组高(P <0 .0 5)。高脂饲料可致心肌及肝脏组织NO含量降低但却升高血清及肾脏组织NO含量。单独使用硒或维生素E或二者合用可不同程度降低高脂饲料所致的MDA含量升高 (P <0 .0 5或P <0 .0 1 ) ,也能不同程度地增加心、肝、肾及血清的NO含量 (P <0 .0 5或P <0 .0 1 ) ,合用比单独使用作用更明显。结论 :硒和维生素E单独或联合应用可降低高脂血症大鼠血脂升高所致的脂质过氧化增强作用 。AIM: To investigate the effects of selenium (Se) and vitamin E (VE) in combination and separateness on the peroxidation of hearts, livers, kid neys and serum in rats with hyperlipidemia. METHODS: High-fat- diet (HFD) was used to cause experimental hyperlipidemia rats. 50 SD rats were d ivided into 5 groups: control group; high-fat-diet (HFD) group; HFD+ 0.4 mg·kg -1 Se; HFD+250 mg·kg -1 VE; HFD+ 0.4 mg·kg -1 Se+ 250 mg·kg -1 VE, respectively. After 4 weeks, the MDA contents in hear ts, livers, kidneys and serum were measured through thiobarbituric acid (TBA) me thod. The NO contents in these samples were assayed by NO kits. RESULTS : HFD caused hyperlipidemia, in comparison with the control group, trigl yceride (TG) and very low density lipoprotein (VLDL-C) increased(P< 0.05 ),so did total cholesterol and low density lipoprotein (P < 0.01 ). Meanwhile, HFD strengthened the peroxidation in all of the tissues or serum (P<0.05). NO contents were reduced in hearts and livers, but in creased in serum and kidneys by HFD. VE or Se in combination and separateness d id fight against these effects (P< 0.05 or P < 0.01 ). Meanwhile, VE and/or Se can increase the NO contents in all the exp erimental tissues, combined use of Se and VE are more effective. CONCLUS ION: Se and/or VE can decrease the peroxidation-related damage and modi fy the NO contents in experimental hyperlipidemia rats.

关 键 词：维生素E 高脂血症 脂质过氧化 丙二醛 一氧化氮 

分 类 号：R965[医药卫生—药理学] R977.25[医药卫生—药学]