再生障碍性贫血造血细胞Fas抗原表达与T淋巴细胞功能的研究  

Studies of Expression of Fas Antigen on Hematopoietic Cells and T Lymphocytic Function in Patients with Aplastic Anemia

作　　者：傅爱林[1] 伍世礼[2] 玄风华[3] 

机构地区：[1]江苏大学附属昆山医院血液肿瘤科,江苏昆山215300 [2]江西医学院第一附属医院血液科,江西南昌330006 [3]江西医学院第一附属医院细胞检测中心,江西南昌330006

出　　处：《江西医学院学报》2004年第1期10-13,共4页Acta Academiae Medicinae Jiangxi

摘　　要：目的　研究造血细胞凋亡与T淋巴细胞免疫在再生障碍性贫血 (再障 ,Aplasticanemia ,AA)发病机制中的作用及两者相关性。方法　采用流式细胞仪测定 4 0例AA患者和 15例非血液、免疫系统疾病对照者骨髓单个核细胞 (BMMNC)的总CD3 4 + 、CD3 4 + Fas+ 、CD3 4 + Fas-、CD4+ 、CD8+ 、CD3 + 、CD3 + CD2 5+ 标记值。结果　 (1)与对照组比较 ,AA组总CD3 4 + 细胞 %明显减少而其Fas表达率 (以占总CD3 4 + 细胞 %为计 )明显增高。 (2 )AA组CD3 4 + 细胞 %数与其Fas抗原表达率无明显负相关。 (3)AA组T细胞 %明显增多 ,且以CD8+ 细胞和CD3 + CD2 5+ 细胞增多为主。 (4 )AA组CD3 4 + 细胞 %数与其T细胞活化状态无显著负相关。 (5 )AA组CD3 4 + 细胞Fas表达率与其T细胞活化状态无显著正相关。结论　AA骨髓存在着造血细胞数量减少和T淋巴细胞亚群数量、功能的异常 ,造血细胞数量减少还可能与Fas以外途径诱导的凋亡过度有关。骨髓造血细胞凋亡过度可能有活化T淋巴细胞免疫以外的途径诱导Fas途径或活化T淋巴细胞可以通过Fas之外的途径诱导造血细胞凋亡。Objective To evaluate the effects of hematopoietic cells apoptosis and T lymphatic cellular immune function on the pathogenesis of aplastic anemia(AA)and their correlations.Methods Expression of Fas antigen on CD 34 + cells and T lymphocyte subsets,CD 25 + antigen on CD 3 + cells in the bone marrow mononuclear cells in 40 AA patients(CAA 24,AAA 16)and 15 normal controls were assayed by using flow cytometry.Results:CD 34 + cells and CD 4 +/CD 8 + ratio were lower,and CD 34 + Fas + cells,CD 8 + cells,CD 33 + cells,CD 3 +CD 25 +cells were higher in AA patients than that in normal controls.Every index value of them in AA patients,except CD 3 +cells and CD 4 +cells only in CAA patients,showed significant difference in comparison to normal control.Respectively,CD 34 +cells level. CD 34 + Fas + cells level was no markedly lineally positive or negative correlated with CD 3 + and CD 25 + cells value.CD 34 + cells and CD 8 + cells were higher,and CD 34 +Fas +cells,CD 3 +cells,CD 3 +CD 25 +cells were lower in CAA patients than in AAA ones,however,there was no significant difference between two groups.Conclusion The decreased numbers of CD 34 +cells in the bone marrow related to Fas-mediated overapoptosis of themselves may have been implicated in pathogenesis of AA.There may be other pathways by which to cause the deficiency of CD 34 +cells, besides Fas-mediated apoptosis pathway.The abnormity in T lymphocyte subsets and their function may have played an important role in the pathophysiology of immune-mediated AA.Overapoptosis of progenitors and stem cells were induced by more than activated T lymphocyte-mediated pathway. In addition to Fas-triggered apoptosis,other pathways may have been suggested as an effector mechanism by which T lymphocyte eliminate their targets.

关 键 词：再生障碍性贫血 造血细胞 FAS抗原 T淋巴细胞 

分 类 号：R556.5[医药卫生—血液循环系统疾病]