鱼腥草素钠与血清白蛋白结合常数的电泳法测定  

Determination of the Binding Constant Between Sodium Houttuyfonate and Human Serum Albumin by Capillary Electrophoresis

作　　者：连春霞[1] 甘婷婷[1] 陈华[1] 袁佩[2] 夏之宁[1] 

机构地区：[1]重庆大学化学化工学院,重庆400030 [2]重庆医科大学药学系,重庆400016

出　　处：《重庆大学学报（自然科学版）》2004年第2期59-62,共4页Journal of Chongqing University

基　　金：国家自然科学基金资助项目(20175038);教育部"高校青年教师奖"资助项目(教人司[2002]123)

摘　　要：采用毛细管电泳峰漂移法测定了鱼腥草素钠(SH)与人血清白蛋白(HSA)在碱性缓冲液条件下的结合常数。用3种求解结合常数的方法,即双倒数法、y-倒数法、x-倒数法,进行数据处理,求得的结合常数分别为4.072×104、3.845×104和3.791×104L·mol-1。SH与HSA结合较强,结合比为1∶1,此结合参数为进一步研究SH在体内的运输、分布以及活性提供了重要信息。The binding constant between sodium houttyfonate(SH) and human serum albumin(HSA) was determined (using) the mobility shift model of capillary electrophoresis interaction analysis. Three different linear plotting methods, namely double reciprocal, y-reciprocal and x-reciprocal were used to measure the binding constant, 4.072×10^(4),(3.845×10^(4)) and 3.791×10^(4) L/mol were obtained respectively. These results confirm that the interaction is strong, which can provide important information for further studying the transportation, distribution and activity of SH in human bodies.

关 键 词：鱼腥草素钠 人血清白蛋白 结合常数 毛细管电泳 

分 类 号：O658[理学—分析化学]