Cloning and expression of ODF /OPGL /RANKL gene related to osseous absorption disease  

作　　者：陶惠人 王全平 张莹莹 刘继中 李靖 

出　　处：《中国临床康复》2002年第20期3128-3129,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：Objective To clone,express and purify recombinant OPG/RANKL /TRANCE.Method RNAwas extracted from the fetal live r and was reverse transcribed into cDNA.RANKL gene was amplified by RT-PCR with designed primers and cDNAas tem plate.The product of PCR were cloned into His-tagged express ion vector-pProEX TM Thc and sent to sequence.Induced by I PTG,recombinant protein of the RANKL was ex-pressed in Escherichia coli stably.The protein was purified by Niion exchange colum.Result Weight of PCR product was 500bp and se quence of it were correct.Molucular weight of recombinant protein was 20kD.Conclusion ODF /OPGL /RANKL /TRANCE gene can be a mplified simply and conveniently by RT-PCR from the c DNAof fetal liver.Recombinant RANKL protein could be obtained by inducing Prokaryotic expression of it.Objective To clone,express and purify recombinant OPG/RANKL /TRANCE.Method RNAwas extracted from the fetal live r and was reverse transcribed into cDNA.RANKL gene was amplified by RT-PCR with designed primers and cDNAas tem plate.The product of PCR were cloned into His-tagged express ion vector-pProEX TM Thc and sent to sequence.Induced by I PTG,recombinant protein of the RANKL was ex-pressed in Escherichia coli stably.The protein was purified by Niion exchange colum.Result Weight of PCR product was 500bp and se quence of it were correct.Molucular weight of recombinant protein was 20kD.Conclusion ODF /OPGL /RANKL /TRANCE gene can be a mplified simply and conveniently by RT-PCR from the c DNAof fetal liver.Recombinant RANKL protein could be obtained by inducing Prokaryotic expression of it.

关 键 词：骨吸收性疾病 破骨细胞分化因子 克隆 重组蛋白 

分 类 号：R68[医药卫生—骨科学]