模拟失重对大鼠比目鱼肌肌梭钙结合蛋白D-28K免疫反应性的影响(英文)  

Effects of Simulated Weightlessness on Calbindin D-28K-immunoreactivity in Rat Soleus Muscle Spindle

作　　者：吴苏娣[1] 樊小力 唐斌 

机构地区：[1]西安交通大学医学院生理教研室,陕西西安710061

出　　处：《航天医学与医学工程》2002年第5期313-316,共4页Space Medicine & Medical Engineering

基　　金：SupportedbytheNationalScienceFoundationofChina(No .3 0 0 70 83 0 )

摘　　要：目的检查模拟失重对大鼠比目鱼肌肌梭钙结合蛋白D 2 8K样免疫反应阳性物质 (CaBP LI)的影响 ,探讨模拟失重所致肌梭损伤的发生机制。方法采用免疫过氧化酶反应ABC法观察尾吊 7d、1 4d与对照组大鼠比目鱼肌梭内肌纤维CaBP LI的差别。结果梭外肌纤维和神经纤维不显示CaBP免疫反应性。每个肌梭都有某些梭内肌纤维上发现CaBP LI。悬吊 1 4d后 ,梭内肌纤维的CaBP免疫反应性明显降低。结论模拟失重可致大鼠比目鱼肌肌梭的CaBP免疫反应性发生改变 ,可能与肌梭的退行性改变有关。Objective To analyze the mechanism involved in muscle spindle deterioration during simulated weightlessness. Method Using the immunoperoxidase reaction utilizing the ABC (avidin biotin complex) method,Calbindin D28K like immunoreactivity(CaBP LI) of intrafusal fibres in soleus muscle were detected in 7 d,14 d tail suspended rats and compared with control rats. Result The extrafusal muscle fibres and nerve fibres did not exhibit immunoreactivity to CaBP. CaBP LI was found in some of the intrafusal muscle fibres in all the muscle spindles. After 14 d suspension, the immunoreactivity to CaBP of the intrafusal fibres decreased markedly. Conclusion Simulated weightlessness could induce changes in the immunoreactivity of rat soleus muscle spindle to CaBP, which may contribute to the deterioration of muscle spindle.

关 键 词：模拟失重 钙结合蛋白 比目鱼肌 肌梭 免疫反应性 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]