Expression and cellular localization of interleukin 8 mRNA and protein in the area of xenogenic bone implant  

Expression and celular localization of interleukin-8 mRNA and prote in in the area of xenogenic bone implant

作　　者：徐虎 胡蕴玉 曹师锋 

出　　处：《Chinese Journal of Traumatology》1999年第1期3-7,共5页中华创伤杂志（英文版）

摘　　要：Objective: To observe the expression and cellular localization of interleukin 8 (IL 8) mRNA and protein in the area of xenogenic bone implant. Methods: The bovine cancellous bone granules were implanted into the thigh muscles of mice. The samples were taken 4, 7, 14 and 21 days after implantation. IL 8mRNA and protein in the site of implant were assayed by in situ hybridization and immunocytochemical techniques. Results: The expression of IL 8mRNA and protein were observed in all specimens 4, 7, 14 and 21 days after implantation. IL 8mRNA was expressed mainly by the neutrophils, monocytes, macrophages and fibroblasts at 7th day post implantation. Some mesenchymal cells, multinucleated giant cells, vascular endothelial cells and smooth muscle cells also expressed IL 8mRNA in the area of xenogenic bone implant at 14th and 21st days. Immunocytochemical studies demonstrated the same results as that of in situ hybridization. Conclusions: Many different kinds of cells express IL 8mRNA and secret IL 8 in the area of xenogenic bone implant, suggesting that IL 8 may play an important role in local immunity of xenogenic bone graft.

关 键 词：Transplantation heterologous  Bone  Interleukin  8  RNA messenger  Proteins  Chin J Traumatol (Eng Ed) 1999 2(1):3  7 

分 类 号：R687.34[医药卫生—骨科学]