Cnstruction of a eukaryote expression vector containing bone morphogenetic protein-2 mature petptide by SOE-PCR method  

出　　处：《中国临床康复》2002年第24期3762-3763,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：Objective To construcl an eukaryote expression uector containing bone morphogenetic protein-2(BMP2)mature peptide,Methods Gene splicing by overlapping extension PCR(SOE-PCR)method was used to clone BMP2 signal peptide and mature peptide and their fusion fragment.The fusion fragment was cloned into an eukaryole expressing vector pc DNA3.1/myc-His(-).A .The sequence of the fusion fragment of BMP2 signal peptide and mature peptide was indentified.Results The sequence of the fusion fragment was correct comparing with BMP2 signal peptide and mature peptide published by NCBI.Cconclusion he vector pcDNA3.1/myc-His(-),A-BMP2 sm constructed in this experiment was suitable to applying in eukaryotic expression of BMP2.

关 键 词：骨形态发生蛋白 载体 成熟肽 基因重叠 PCR 

分 类 号：R68[医药卫生—骨科学]