基于荧光猝灭原理的胆固醇光纤生物传感器的研制  

DEVELOPMENT OF FIBER OPTICAL BIOSENSOR OF CHOLESTEROL BASED ON FLUORESCENCE QUENCHING

作　　者：闫玉华[1] 徐运华[1] 李世普[1] 

机构地区：[1]武汉理工大学生物材料与工程研究中心,武汉430070

出　　处：《中国生物医学工程学报》2004年第1期44-48,共5页Chinese Journal of Biomedical Engineering

基　　金：高等学校博士学科点专项基金项目资助 ( 980 4970 3 )

摘　　要：以荧光猝灭原理为基础 ,以钌Ⅱ 邻菲咯啉为荧光试剂制备荧光薄膜 ,以醋酸纤维素为胆固醇氧化酶固定化载体 ,研制了胆固醇光纤生物传感器。利用相移法原理 ,采用锁相放大技术 ,实现了对胆固醇溶液中溶解氧和胆固醇含量的检测。该传感器具有较高的灵敏度 ,快的响应时间和较好的稳定性。In this work, a fiber optical biosensor for detecting cholesterol was developed based on fluorescence quenching. Ru(phen)32+ was used as a fluorescent reagent to prepare fluorescent membrane, and cellulose acetate was used as a carrier of the immobilized cholesterol oxidase. The dissolved oxygen and cholesterol content in cholesterol solution were detected by means of phase-shifting and phase lock means. This sensor has high sensitivity, Short response time and good stabilization.

关 键 词：光纤传感器 胆固醇 荧光猝灭 相移法 

分 类 号：R318.08[医药卫生—生物医学工程]