不同发育阶段红系造血细胞的流变特性的研究  

RHEOLOGICAL STUDIES OF THE PRECURSOR CELLS AT DIFFERENT STAGES

作　　者：王金华[1] 喀蔚波[1] 孙大公[1] 曾柱[1] 文宗曜[1] 

机构地区：[1]北京大学医学部基础医学物理教研室,北京100083

出　　处：《中国生物医学工程学报》2004年第1期25-29,共5页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学资金 ( 3 983 0 110 );博士点基金资助项目

摘　　要：通过对不同发育阶段红系造血细胞的变形性、取向性和膜的流动性的研究 ,发现它的变形性、取向性和膜的流动性随着分化发育而不断改变 ,而且这种变化以晚幼红细胞为转折点。这些变化与核的不断固缩 ,偏核 ,排核 ,细胞核与细胞质比不断减少 ,膜骨架蛋白 (尤其膜收缩蛋白 )变化及脂质分子与蛋白原分子相互作用有关。本研究还对用电子自旋共振测得结果和用LBY BX2型激光衍射仪测得结果作相关分析 ,表明它们存在负相关关系 ,相关系数R2 =0 .9834。In this work, the deformability and membrane fluidity of precursor cells at different developing stages were investigated. We found that the deformability and membrane fluidity both increased with development of the cells, and normoblast is the turning point in the development of precursor cells. The changes are thought to be relted with the condensing of the nucleus, decrease of the ratio of nucleus to cytoplasm, variation of the membrane skeleton protein, and the interactions of proteins and lipids. Order parameter (S) obtained by ESR is negatively correlation with Deformation index (ID) d,max obtained by LBY-BX2 ektacytometry, the value of R 2(correlation coefficient's square) is 0. 9834. The resats indicate the membrane fluidity is closely related with deformability.

关 键 词：早期红系造血细胞 细胞变形性 细胞取向性 膜的流动性 

分 类 号：R318.01[医药卫生—生物医学工程]