黄芪皂苷抗实验性肝损伤作用  

EFFECTS OF ASTRAGAGALUS(ASI,SK)ON EXPERIMENTAL LIVER INJURY

作　　者：张银娣[1] 沈建平[1] 朱树华[1] 黄大贶[1] 丁勇[1] 张晓林[1] 

机构地区：[1]南京医学院药理教研室,南京210029

出　　处：《药学学报》1992年第6期401-406,共6页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助题编号3870576

摘　　要：两种黄芪苷ASI和SK能对抗D-半乳糖胺,醋氨酚引起的肝损伤。表现为减轻肝毒引起的病变,SGPT降低,肝MDA下降,GSH升高;且三项指标变化呈明显相关。从离体大鼠肝细胞制备法也证明ASI和SK在0.75μmol/L～0.18 nmol/L浓度范围内,可对抗CCl_4引起的GPT升高。给小鼠连续灌服ASI 12,50 mg/kg或SK 6,20 mg/kg能提高肝微粒体细胞色素P—450活性。此结果表明,黄芪苷是黄芪抗肝毒损伤的有效成分。其机理除抗生物氧化外,尚与代谢调节作用有关。The saponins (ASI, SK) used in this study was extracted from the rootof Astragalus membraneceus Bge and Astragalus sieversianus Pull. ASI and SK were foundto protect liver from chemical injury induced by CCl_4, D-galatosamine andacetaminophen in mice. The two saponins were shown to impede the elevation of SGPTlevel, decrease the MDA content and increase the GSH concentration in mouse liver. Obvi-ous improvement of histological changes were also observed. The protective action of ASIand SK against the hepatotoxicity was also shown in experiments using primary culturedrat hepatocytes. The average value of GPT in the medium treated with different concentra-tion of ASI and SK(0.00075 ～0.18 mmol/L )was lower than that in control. Analyzing through multiple linear correlation, we showed that the lowering of SGPT was negativelyrelated to the increase of GSH, positively related to the decrease of MDA in mice givenCCl_4 or acetaminophen in combination with ASI or SK. These results indicate thatthe hepato- protective effects of ASI and SK may be due to their antioxidation activities,since the content of liver protein in mice given ASI or SK was more than that in the con-trols. Morever, the level of hepatic microsomal cytochrome P- 450 in all mice given thetwo saponins were significantly increased, the liver metabolism and immunoregulating ac-tion produced by ASI and SK may be also involved in their hepato- protective effects.

关 键 词：黄芪皂苷甲 绵毛黄芪苷 肝炎 

分 类 号：R975.5[医药卫生—药品]