四环素类抗生素的RP-HPLC分析  

REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF TETRACYCLINES

作　　者：杨大军[1] 张瑞[1] 

机构地区：[1]中国医学科学院医药生物技术研究所,北京100050

出　　处：《药学学报》1992年第7期539-543,共5页Acta Pharmaceutica Sinica

摘　　要：报道了用反相高效波相色谱分析去甲基金霉素等四环素类抗生素和它们的差向异构体以及其脱水物的方法。采用Water 10C_(18) 4.6mm ID×259mm的色谱柱,以14％乙腈-3％二甲基甲酰胺-83％0.02mol/L柠檬酸水溶液为流动相,流速1.0ml/min,UV 350 um检测,可将四环素、去甲基四环素、金霉素、去甲基金霉素和它们的差向异构体八种化合物完全分离;以15％乙腈-15％二甲基甲酰胺-70％ 0.02mol/L柠檬酸水溶液为流动相,流速1.0ml/min,UV270nm检测,可将上述抗生素及其差向异构体以及它们的脱水物十六种化合物分离。它们的发酵液经简单处理亦可直接进样分析。同时还考查了pH、溶剂等因素对分析的影响。The present paper describes a quantitative determination method oftetracyclines by reversed phase high performance liquid chromatography. The method used a Waters 10 C_(18) 4. 6 nm ID×250 nm reversed phase column, witha mobile phase composed of 14% acetonitrile-3% dimethylformamide-83% water con-tining 0.02 mol/L citric acid (pH≈2.5), the detector wavelength was set at 350 nm, andthe flow rate was maintained at 1.0 ml/min. Demethylchlortetracycline, epi-demethylchlortetracycline, demethyltetracycline, epi-demethyltetracycline, tetracycline, epi-tetracycline,chlortetracycline and epi-chlortetracycline can be separated highly efficiently. Thetetracyclines and their anhydro-products can be separated on the same column with 15%acetonitrile-15% dimethylformamide-70% O. 02 mol/L citric acid as the mobile phase,and detected at UV 270 nm.

关 键 词：四环素 去甲基金霉素 HPLC 

分 类 号：R978.14[医药卫生—药品]