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机构地区:[1]华西医科大学药学院天然药物化学教研室,成都610041
出 处:《药学学报》1992年第11期849-852,共4页Acta Pharmaceutica Sinica
摘 要:采用ODS柱,以甲醇—水(2:8)为流动相,在276nm检测波长下,测定了大花红景天、互生红景天、喜马红景天、云南红景天和四裂红景天5种生药以及大花红景天提取物10个样品中的红景天甙的含量。This paper reports a high performance liquid chromatographic (PHLC) method for the determination of salidroside in 10 samples of the plants of Rhodiola crenulata, R. alterna, R. breripetiola- ta, R. yunnanensis and R. quadrifida as well as extracts of R. crenulata. Determination was carried out under the following conditions: column (μBondapak ODS, 10 μm, 3. 9 mmx300 mm), mobile phase (methanoi--water, 2:8), flow rate (1.0 ml/min), detector (Waters 484, wavelength 276 nm). External stadard method was used. The results show that HPLC is a useful tool for the quantitative analysis of salidroside in the plants of Rhodiola genus and the extracts of R. crenulata.
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