脂质过氧化诱导动脉粥样硬化形成与纤连蛋白及P105基因的关系(英文)  

The relationships between lipid peroxide inducing atheromatous formation and fibronectin and gene P105

作　　者：李咏梅[1] 厉曙光 宋伯根[1] 

机构地区：[1]同济大学医学院病理解剖学教研室,上海市200331 [2]同济大学医学院预防医学教研室,上海市200331

出　　处：《中国临床康复》2004年第6期1152-1153,F003,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:初步研究阿霉素导致血管壁脂质过氧化状态下纤连蛋白(fibronectin,FN)及P105基因表达的变化及其意义,并对动脉粥样硬化患者的临床康复介入提供理论依据。方法:将雄性SD大鼠40只随机分成4组,每组10只:正常对照组,阿霉素低剂量组(10mg/kg),阿霉素中剂量组(20mg/kg),阿霉素高剂量组(40mg/kg),对后3组分别一次性腹腔注射不同剂量的阿霉素,制备脂质过氧化模型。采用硫代巴比妥酸(Thiobarbituricacid,TBA)荧光分光光度法测定大鼠血清脂质过氧化产物丙二醛含量。运用反转录聚合酶链反应(RT-PCR)测定大鼠主动脉中的纤连蛋白(Fibronectin,FN)、P105共活化因子mRNA的表达。结果:对照组、阿霉素低、中、高剂量组大鼠血清丙二醛的含量分别为(12.6±3.8),(17.4±2.9),(20.9±3.4),(24.5±4.1)μmol/L,其中中、高剂量组大鼠血清中丙二醛含量高于对照组(t=2.27,P<0.05;t=2.89,P<0.01);RT-PCR结果表明FN,P105mRNA在不同剂量模型组呈不同程度的高表达。结论:在阿霉素导致血管壁损伤后,有FN,P105基因的高表达,脂质过氧可能通过多种途径诱导动脉粥样硬化的形成。AIM:To provide a theoretical gist in clinical interventions on atherosclerotics by an elementary investigation on the alterations and the significance of fibronectin(FN) under lipid peroxide condition of the blood vascular wall induced by adriamycin(ADR) and the expression of gene P105. METHODS:Forty male SD rats were randomly allocated into 4 groups with 10 cases each:control group,ADR low dose group(10 mg/kg),ADR medium dose group(20 mg/kg) and ADR high dose group(40 mg/kg). Adriamycin of different doses was injected one off into the abdominal cavities of the rats in the latter three groups respectively for the preparation of lipid peroxide model.The contents of malondialdehyde(MDA),the product of lipid peroxide,were detected by thiobarbituric acid(TBA) spectrophotofluorimetry.FN and mRNA of P105 gene in the aorta of the rats were examined by RT PCR. RESULTS: The results of serous MDA contents in the control group and low dose,medium dose and high dose ADR groups were( 12.6± 3.8) , ( 17.4± 2.9) , ( 20.9± 3.4) , ( 24.5± 4.1) μ mol/L respectively in rats,in which,the contents in medium and high dose groups were significantly higher than those of control group(t=2.27,P< 0.05; t=2.89, P< 0.01).The results of RT PCR indicated that there were high expressions of FN and P105 mRNA with different degrees in different dose groups. CONCLUSION: The high expressions of FN and P105 gene after the vascular damaged by ADR and lipid peroxide can induce atheromatous formation through multiple approaches.

关 键 词：脂质过氧化 动脉粥样硬化 纤连蛋白 基因表达 P105基因 康复 

分 类 号：R543.5[医药卫生—心血管疾病] R394[医药卫生—内科学]