双下肢缺血后处理对大鼠心肌缺血再灌注损伤的保护作用  

An Experimental Study on Protective Effects of Non-wound IschemicPostconditioning to Ischemia/Reperfusion Myocardial Injury of Rats

作　　者：刘兴德[1] 袁志柳[2] 黄达枚[1] 

机构地区：[1]贵阳医学院附院心内科,贵州贵阳550004 [2]贵州省人民医院干医科,贵州贵阳550002

出　　处：《贵阳医学院学报》2004年第1期7-9,共3页Journal of Guiyang Medical College

摘　　要：目的 :探讨在体条件下 ,非创伤性双下肢缺血后处理 (N WIPTC)是否具有减轻心肌缺血再灌注 (I/R)损伤的作用。方法 :采用SD大鼠心肌I/R模型 ,应用Evan蓝、TTC染色 ,观察N WIPTC对心肌梗死面积的影响 ;并应用生化检测及免疫组织化学技术 ,研究N WIPTC、丙二醛 (MDA)、超氧化物歧化酶 (SOD)对大鼠心肌I/R损伤的作用。结果 :与I/R组比较 ,N WIPTC组的心肌梗死面积减少 ,MDA含量显著降低 (P <0 .0 1 ) ,而SOD含量明显升高 (P <0 .0 1 )。结论 :N WIPTC能有效降低心肌I/R梗塞面积 ,其机制可能与减少自由基损伤和抗氧化作用加强有关。Objective: To observe if there would be protective effects of ischemic postconditioning in non wound legs (N WIPTC) on ischemia/reperfusion (I/R) caused mycocardia injury of rats in vivo. Methods: 30 Sprague Dawley rats were divided randomly into four groups and the model of I/R myocardial injury were produced. The influence of N WIPTC on infarction sizes (IS) was measured with Evan’s and TTC dye. The effects of N WIPTC, malondialehyde (MDA) and superoxide dismutase (SOD) on the I/R caused mycocardia injury were tested with biochemistry and immunohistochemistry techniques. Results: Compared with those of I/R group, the IS and the concentration of MDA in plasma of N WIPTC group were lower (P<0 01), and the concentration of SOD in plasma of N WIPTC group was higher. Comparing with that of the ischemic preconditioning (IPC) group, the IS and the concentrations of MDA and SOD in plasma were not significantly different between IPC group and N WIPTC group (P>0 05). Conclusion: N WIPTC has the same protective effects as IPC through decreasing the IS. The potential mechanism might be decreasing of the injury caused by oxygen free radical and strengthening the action of antioxidization.

关 键 词：心肌再灌注损伤 心肌梗塞 大鼠 

分 类 号：R542.220.1[医药卫生—心血管疾病]