碳-13同位素示踪结合^(13)C-NMR分析稻杆木素结构  

The Structure of Rice Stalk Lignin Analyzed by Tracer Isotope Carbon 13 and Carbon 13 Nuclear Magnetic Resonance

作　　者：杨海涛[1] 谢益民[1] 杨跃生[2] 

机构地区：[1]制浆造纸工程国家重点实验室(华南理工大学),广州510640 [2]华南农业大学生命科学学院,广州510640

出　　处：《造纸科学与技术》2004年第1期1-5,共5页Paper Science & Technology

基　　金：国家自然科学基金(299740 11;3 0 170 756);教育部博士点基金(2 0 0 2 0 5610 2 2 );教育部重点项目基金 (0 113 7);广东省教育厅"千;百;十工程"优秀人才培养基金资助项目

摘　　要：为了进一步了解单子叶植物的木素 ,向生长中的水稻 (OryzasativaL .)茎秆节间的腔体分别注入在侧链α、β、γ位带13 C标记的松柏醇葡萄糖甙 ,得到侧链带13 C标记的稻秆木素。通过CP/MAS13 C NMR分析 ,发现外源性的松柏醇葡萄糖甙并不影响水稻中木素和碳水化合物的正常生物合成。液态13 C NMR检测结果证明稻秆原本木素的主要结构是 β O 4、β 1、β β和 β 5结构 ,还有少量的松柏醇和α O 4结构。另外 。To further reveal the structure of monocotyledon lignin, carbon 13 enrichment of specific carbons of rice stalk lignin was achieved by feeding coniferin [α 13 C], coniferin [β 13 C] and coniferin [γ 13 C] into an internode cavity of the stalk of a growing rice plant( Oryza sativa L.). The resulting rice stalk tissues were analyzed by solid state CP/MAS 13 C NMR spectroscopy. It was found that the administration of coniferin did not affect the normal ligninfication of rice plant and the normal formation of carbohydrate of rice stalk. Furthermore, high resolution 13 C NMR spectra of milled lignin from rice stalk were also determined. It was also proved thatβ O 4?β 1?β βandβ 5 were main substructure in protolignin of rice stalk although there were some minor substructures as coniferyl alcohol andα O 4 unit. Importantly, the result indicated that significant bonding occurred at α position to other constitutes, presumed to be mainly of carbohydrates.

关 键 词：碳-13同位素示踪 ^13C-NMR 稻杆 木素 木素-碳水化合物复合体 化学结构 

分 类 号：TS714[轻工技术与工程—制浆造纸工程]