拮新康逆转K562/A02细胞多药耐药的机理研究  

The Mechanism of Chinese Medicine Compound Jiexinkang to Reverse the Multidrug Resistance of K562/A02 Cells

作　　者：黄程辉[1] 谢兆霞[1] 秦群[1] 林秀梅[1] 

机构地区：[1]中南大学湘雅医院,湖南长沙410008

出　　处：《湖南中医学院学报》2004年第1期7-10,共4页Journal of Hunan College of Traditional Chinese Medicine

基　　金：湖南省科技厅重点资助项目 [97SSY0 0 1( 3 ) ]。

摘　　要：目的利用血清药理学方法探讨中药拮新康复方逆转K5 62 /A0 2细胞多药耐药的机理 ,为临床应用提供理论和实验依据。方法分别采用MTT法、RT—PCR、Western印迹法观察含拮新康大鼠血清处理后K5 62 /A0 2细胞的增殖及mdr—1 /P—gp表达的改变。结果含 1 0 %拮新康血清 +ADM组对K5 62 /A0 2细胞的抑制率比 1 0 %正常大鼠血清 +ADM组明显增加 (P <0 .0 5 ) ;含 1 0 %拮新康血清 +ADM组K5 62 /A0 2细胞的mdr—1及P—gp的表达比ADM组及 1 0 %正常大鼠血清 +ADM组均降低 (P <0 .0 1 )。结论拮新康能抑制K5 62 /A0 2细胞的增殖 ,通过降低mdr—1 /P—gp的表达逆转K5 62 /A0 2细胞的多药耐药。Objective To investigate the mechanism of Chinese medicine compound Jiexinkang to reverse the multidrug resistance of K562/A02 cells by using the procedures of serumpharmacology so as to provide academic and experimental foundation for clinical application.Methods The procedures of MTT method, reverse transcription polymerase chain reaction and Western blotting were used respectively to determine the alteration of proliferating activity and mdr 1/ P gp expression of K562/A02 cells after being treated with rat serum containing Jiexinkang. Results The inhibiting rate to K562/A02 cells with 10% rat serum containing Jiexinkang plus adriamycin (50 μg/mL) was significantly higher than that of 10% normal rat serum containing adriamycin (50?μg/mL) alone( P <0.05). The mdr 1/P gp expression of K562/A02 cells treated with 10% rat serum containing Jiexinkang plus adriamycin (50?μg/mL) was significantly lower than that with adriamycin (50?μg/mL) and that with 10% normal rat serum containing adriamycin (50?μg/mL) respectively( P <0.01). Conclusion Jiexinkang can inhibit the proliferation of K562/A02 cells and reverse multidrug resistance of K562/A02 cells by down lregulating mdr 1/P gp expression.

关 键 词：拮新康 莲子心 黄芪 K562/A02细胞 血清药理学 P-糖蛋白 多药耐药 

分 类 号：R285.5[医药卫生—中药学]