苏云金杆菌杀虫晶体蛋白基因cry1A的克隆及特征分析  

Cloning and characterization analysis of insecticidal crystal protein gene cry1 A from Bacillus thuringiensis

作　　者：钟万芳[1] 蔡平钟[1] 阎文昭[1] 裴炎[2] 

机构地区：[1]四川省农业科学院生物技术育种中心,四川成都610066 [2]西南农业大学生物技术中心,重庆北碚400716

出　　处：《西南农业学报》2004年第1期38-40,共3页Southwest China Journal of Agricultural Sciences

基　　金：四川省生物技术重点资助项目

摘　　要：苏云金芽孢杆菌猝倒亚种(Bacillusthuringiensissubsp.sotto)对鳞翅目幼虫有高毒力。根据cry1A类基因序列设计特异引物,应用PCR技术从该菌株中扩增得到一大小约为3 6kb的DNA片段。将获得的片段克隆至pGEM7zf中并转化大肠杆菌DH5α。在ABIPRISM377自动测序仪上对其5′及3′端进行部分测序,结果表明其核苷酸序列与cry1A基因高度同源。Bacillus thuringiensis subsp.sotto is highly toxic to lepidopetera larvae.Using computer analysis of cry1A gene sequences,a pair of specific primers was designed.With the primers,PCR was performed and about 3.6kb fragments were obtained from Bacillus thuringiensis subsp.sotto.The fragments were ligated into the cloning vector pGEM7zf and then transformed into E.coli DH5α.Partial sequencing was done to the 5′-end and 3′-end by ABI PRISM 377 DNA suquencer.The result revealed its high homology with cry1A genes.

关 键 词：苏云金芽孢杆菌 杀虫晶体蛋白基因 序列分析 克隆 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]