根癌农杆菌介导的豇豆胰蛋白酶抑制剂基因转化芥菜的研究  

Transforming Brassica juncea Coss with cowpea trypsininhibitor gene mediated by Agrobacterium tumefaciens

作　　者：杨朝辉[1] 何凤田[1] 宋明[2] 江渝[1] 彭家和[1] 李蓉芬[1] 黄刚[1] 

机构地区：[1]第三军医大学生物化学与分子生物学研究室,重庆400038 [2]西南农业大学园艺系,重庆400716

出　　处：《西南农业学报》2004年第1期74-77,共4页Southwest China Journal of Agricultural Sciences

摘　　要：为得到具有较强抗虫性的新的芥菜种质资源,用农杆菌介导将豇豆胰蛋白酶抑制剂(CpTI)基因导入芥菜,获得了Kan抗性植株。经PCR扩增、PCR Southernblot和Northernblot分析,转化再生植株大部分呈阳性,而非转化的再生植株均为阴性,证明CpTI基因已存在于芥菜基因组中。在室内进行了喂虫试验,结果表明转基因芥菜抗虫性明显高于对照,转基因植株之间存在抗虫性差异。Explants of mustard were transformed with Agrobacterium tumefaciens (LBA4404) to get new source of insect-resistant mustards.The putative transformation regeneration plants were assayed by PCR,PCR-Southern blot and Northern blot analysis.By these analyses,the target bands were observed,the integration of the Cowpea trypsin inhibitor(CpTI)gene into mustard genome DNA was confirmed.The result of insect-resistance showed that the transgenic plants were more resistant than non-transgenic plants.

关 键 词：豇豆胰蛋白酶抑制剂基因 芥菜 转化 抗虫 

分 类 号：S637[农业科学—蔬菜学]