抗纤软肝颗粒对肝星状细胞凋亡的影响  

Effects of Kangxian Ruangan Granule(KXRG) on Hepatic Stellate Cells Apoptosis

作　　者：杨玲[1] 张赤志[2] 

机构地区：[1]华中科技大学同济医学院协和医院中医科,湖北武汉430022 [2]湖北中医学院附属医院

出　　处：《中西医结合肝病杂志》2004年第1期19-22,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：湖北省自然科学基金课题 (No :2 0 0 0J0 4 2 );湖北省教育厅科研基金课题 (No :2 0 0 0A0 60 1 0 )

摘　　要：目的 :探讨抗纤软肝颗粒对肝星状细胞 (HSC)凋亡的影响。方法 :传代培养的大鼠肝星状细胞系HSC T6与中药复方抗纤软肝颗粒 (终浓度为 5mg/ml、 2 5mg/ml、 1 2 5mg/ml)及药物血清 ( 5 %、 10 %、 2 0 % )共同培养48小时后 ,应用TUNEL法及流式细胞仪测定细胞凋亡、免疫组化检测Bcl 2 /Bax。结果 :TUNEL法及流式细胞仪测定结果显示抗纤软肝颗粒药物及含药血清均可诱导HSC凋亡 (P <0 0 5 ) ;并能下调凋亡抑制分子Bcl 2 ,上调促凋亡分子Bax (P <0 0 1)。结论 :抗纤软肝颗粒可下调Bcl 2 /Bax比率 ,以增加细胞对凋亡的敏感性 ,从而促进HSC凋亡。Objective:To investigate the role of KXRG in inducing apoptosis and its mechanism. Methods:The cell apoptosis was analyzed by flow cytometry, and with TUNEL method. The expression of Bax、Bcl-2 was detected with cell immunocytochemistry stain. Results:In apoptosis both drug and medicated sera of KXRG could induce apoptosis (P<0.05), down regulate the expression of Bcl-2, a molecular of cell survival, and up-regulate the expression ofBax, a death-promoting molecule(P<0.01).Conclusion: KXRG could down regulate the ratio of Bcl/Bax to increase the sensitivity of apoptosis and induced HSC apoptosis.

关 键 词：抗纤软肝颗粒 肝星状细胞 细胞凋亡 TUNEL法 流式细胞仪 中医药疗法 肝纤维化 

分 类 号：R285[医药卫生—中药学]