极端污染环境下草苷膦抗性菌株HTG7的筛选及其特性研究  

SEPARATION AND CHARACTERIZATION OF GLYPHOSATE-TOLERANT BACTERIAL STRAIN HTG7 FROM EXTREMELY POLLUTED ENVIRONMENT

作　　者：刘柱[1] 梁爱敏[2] 张维[2] 平淑珍[2] 陈明[2] 陆伟[2] 徐玉泉[2] 杨志荣[1] 林敏[2] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《微生物学通报》2004年第1期35-39,共5页Microbiology China

基　　金：国家高技术研究发展计划项目 ("863"项目 ) (No 2 0 0 1AA2 1 42 3 1 );国家转基因植物研究与产业化专项资助 (No JOO A 0 1 0 )~~

摘　　要：从被草苷膦极度污染的土壤中分离到一株极端抗草苷膦菌HTG7,经初步鉴定其为可变盐单胞菌 (Halomonasvarabilis) ,该菌株最高可以耐受 5 0 0mmol L左右的草苷膦浓度。电镜观察表明 ,5 5 0mmol L的草苷膦浓度下 ,细胞大量坏死。生理生化特性表明该菌最适pH为 7 0 ,并且在氯化钠浓度 0～ 1 0 %生长良好。Bacterial strain HTG7 was isolated from extremely glyphosate-polluted soil. It was identified as Halomonas varabilis preliminarily . It could tolerate in 500 mmol/L glyphosate concentration. Micrograph of HTG7 demonstrated that almost no cells lived well in the 550 mmol/L glyphosate concentration. General characterization of strain HTG7 showed that the optimal pH was 7.0. It grew well in the NaCl concentration range from 0 to 10%.

关 键 词：可变盐单胞菌 草苷膦 生物抗性 

分 类 号：S188[农业科学—农业基础科学]