鲎G因子实验法定量测定药用、食用蘑菇多糖中的(1,3)-β-葡聚糖含量(英文)  

THE QUANTIFICATION OF (1,3)-β-GLUCAN IN EDIBLE AND MEDICINAL MUSHROOM POLYSACCHARIDES BY USING LIMULUS G TEST

作　　者：杨晓彤[1] Jennifer M.F.WAN 糜可[2] 冯慧琴[2] Daniel K.O.CHAN 杨庆尧[2] 

机构地区：[1]香港大学理学院动物学系 [2]上海师范大学微生物与免疫学研究所,上海200234

出　　处：《菌物系统》2003年第2期296-302,共7页Mycosystema

基　　金：the Hong Kong Association for Health Care Ltd.

摘　　要：(1,3)--葡聚糖是蘑菇免疫调节和抗肿瘤多糖的核心结构。通过鲎的G因子法测定了从19种药用和食用蘑菇中提取的27个多糖样品中(1,3)--葡聚糖的含量,我们发现(1,3)--葡聚糖普遍存在于这些蘑菇多糖提取物中。但是,(1,3)--葡聚糖的含量随蘑菇的种类和多糖提取的部位不同有着很大的差异,平均值大约占多糖的34.8%。从香菇、裂褶菌、云芝、草菇、灰树花、鸡腿菇和姬菇中提取的9种多糖中(1,3)--葡聚糖含量远高于其余的多糖。适当的纯化可以提高蘑菇多糖中(1,3)--葡聚糖的含量。我们的研究表明鲎G因子法可以用来快速有效地测定蘑菇多糖提取物中的(1,3)--葡聚糖含量。glucan is a core structure in mushroom polysaccharide, which claim to posses anticancerous and immunomodulatory properties. The ability to identify (1, 3)--glucan in mushroom polysaccharide not only provide useful information on the structural composition of the mushroom polysaccharide, but facilitate us to identify the potential anticancerous and immunomodulatory active compounds. Using limulus factor G test, we detected (1, 3)--glucan in 27 polysaccharides extracted from 19 edible or medicinal mushrooms species. The result show that (1, 3)--glucan exists in all mushroom polysaccharide extracts but its content varies extremely depends on the mushroom species or the part of mushroom and the degree of purification. Our data show the mean of (1, 3)--glucan in these mushroom polysaccharides is 34.8%. Nine mushroom polysaccharide extracts from Lentinus edodes, Schizophyllum commune, Coriolus versicolor, Volvariella volvacea, Coprinus comatus, Grifola frondosa, Lyophyllum shimeji have superior (1, 3)--glucan contents than the others. Our study demonstrates that limulus Factor G test is a quick and convenient method for detecting (1, 3)--glucan content in crude mushroom polysaccharide extracts.

关 键 词：担子菌 提取物 测定 

分 类 号：Q946[生物学—植物学]