中药糖微康对糖尿病大鼠肾皮质MMP-9表达的影响  

Effect of TWK on Expression of MMP -9 in Renal Cortex in Streptozotocin - Induced Diabetic Rats

作　　者：林兰[1] 郭力[2] 

机构地区：[1]中国中医研究院广安门医院,北京100053 [2]中国中医研究院

出　　处：《山西中医学院学报》2003年第2期8-10,共3页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：国家"九五"攻关资助项目(No.96-906-07)

摘　　要：目的:观察糖微康对糖尿病肾病(DN)的治疗作用。方法:以STZ诱导糖尿病动物模型,采用免疫组化和RT-PCR检测方法观察糖微康对糖尿病大鼠肾皮质金属蛋白酶(MMP-9)蛋白、基因表达的影响。结果:糖尿病大鼠肾皮质MMP-9免疫染色强度比模型组大鼠明显增强(P<0.01),MMP-9 mRNA水平比未治疗大鼠明显升高(P<0.01)。结论:糖微康可增强糖尿病大鼠肾皮质MMP-9的表达,预防DN的发生和延缓DN的发展。Objective:To study thethe effect of Tangweikang(TWK) on diabetic nephropathy. Methods:To make diabetic nephropathy animal model and to divide the rats into four groups: normal rats, streptozo-tocin rats untreated group, treated with TWK and captoril group. After 12 weeks, the expression of MMP - 9 mRNA and protein in renal cortex were measured by reverse transcription - polymerase chain reaction (RT - PCR)and immunohistochemical reaction respectively. Result:The TWK group's expression of MMP -9 mRNA and protein in renal cortex had increased than the untreated groupv P<0. 01). Conclusion:It is suggested that TWK can increase the expression of MMP - 9mRNA, protect the image of DN and delay the development of DN.

关 键 词：糖尿病肾病 糖微康 金属蛋白酶 

分 类 号：Q786[生物学—分子生物学] R285.5[医药卫生—中药学]