组织工程血管瓣膜研究中的染料介导光氧化固定技术  

Dye-Mediated Photooxidative Immobilization in the Field of Tissue Engineering for Vascular Conduits and Cardiac Valves

作　　者：李胜利[1] 胡盛寿[2] 姚康德[3] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院，北京100037 [2]中国协和医科大学阜外心血管病医院，北京100037 [3]天津大学材料科学与工程学院，天津300072

出　　处：《生物医学工程与临床》2003年第2期117-119,共3页Biomedical Engineering and Clinical Medicine

摘　　要：心血管外科中应用的异种生物组织一般都要进行固定 ,最为成熟和广泛的方法是戊二醛固定 ,但是其存在很多的缺点 ,具有生物毒性 ,容易钙化 ,耐久性差 ,促使人们努力寻找一种更好的替代方法 ,其中染料介导的光氧化固定被认为是一种很有前途的方法。染料介导光氧化固定方法比较简单 ,把需要固定的组织浸泡于含有光敏染料的缓冲液中光照。染料介导光氧化固定方法相比戊二醛固定 ,具有生物毒性低 ,不易钙化的优点 ,与戊二醛固定一样 ,具有生物稳定性高、免疫原性低的特点。从目前的研究看 。Since the glutaraldehyde fixation has been the ripe and widely applicable technique in treating biomaterials for xenograft,but there are some shortcomings ig. cytotoxic,less durability and easy calcification. Thus the alternative technique should be further developed. The dye-mediated photooxidation immobilization is one of the alternatives and it is considered as a prospective technique. This technique is rather easy and convenient,only need to immerse the biological tissue in a chilled buffer solution contained photoactive dye and expose to a light source with wide spectrum of wave length. In comparing with glutaraldehyde this dye-mediated photooxidation technique is less cytotoxic and calcific,besides the highly biological immobilization and low immunogenicity. The dye-mediated photooxidation is a promising method to immobilize biomaterial for application in cardiovascular bioprosthesis.

关 键 词：光氧化 戊二醛 组织工程 血管瓣膜 

分 类 号：R318.1[医药卫生—生物医学工程]