Establishment of widely expressed hb1f transgenic mouse lineage and its morphologic analysis  

作　　者：WANGShuiliang HEYan XIEYouhua WANGYuan LIJianzhong WANGLong WANGZhugang FUJiliang 

机构地区：[1]DepartmentofMedicalGenetics.SecondMilitaryMedicalUniversity.Shanghai200433,China [2]InstituteofBiochemistryandCellBiology,ShanghaiInstitutesforBiologicalSciences,ChineseAcademyofSciences,Shanghai200031,China [3]ShanghaiNanfangResearchCenterforModelOrganisms.Shanghai201203.China

出　　处：《Chinese Science Bulletin》2004年第2期142-145,共4页

摘　　要：Human nuclear receptor hB1F is a novel member of the fushi tarazu factor Ⅰ subfamily of nuclear receptor superfamily. The studies about its homologous genes indicate that hB1F may play a key role in regulating the metabolic homeostasis of cholesterol. After obtaining the founder mice carrying the trausgeue of hblf by microiujectiou, each founder was mated to normal C57 mouse and the positive F1 by PCR identification of the same founder were iutercrossed within sisters and brothers to establish the trausgeuic mouse lineage. The results of F1, F2 and offspring of test cross identification showed that the widely expressed hb1f trausgeuic mouse lineage was established successfully in this study. The tissue morphology of the trausgeuic lineage was also analyzed preliminarily.Human nuclear receptor hB1F is a novel member of the fushi tarazu factor Ⅰ subfamily of nuclear receptor superfamily. The studies about its homologous genes indicate that hB1F may play a key role in regulating the metabolic homeostasis of cholesterol. After obtaining the founder mice carrying the transgene of hb1f by microinjection, each founder was mated to normal C57 mouse and the positive F1 by PCR identification of the same founder were intercrossed within sisters and brothers to establish the transgenic mouse lineage. The results of Fl, F2 and offspring of test cross identification showed that the widely expressed hb1f transgenic mouse lineage was established successfully in this study. The tissue morphology of the transgenic lineage was also analyzed preliminarily.

关 键 词：脱氧核糖核酸 转基因技术 肝炎病毒 形态学 

分 类 号：R373.2[医药卫生—病原生物学]