用两种方法生产猪丹毒(G4T10)活疫苗的比较试验  

Tests on production of swine erysipelas (G4T10) live vaccine in two ways

作　　者：张金平[1] 孙继强[1] 陶淑清[1] 张明艳[1] 王丽新[1] 涂映霞[1] 

机构地区：[1]黑龙江省生物制品一厂,黑龙江哈尔滨150069

出　　处：《中国兽药杂志》2004年第2期54-55,共2页Chinese Journal of Veterinary Drug

摘　　要：　为提高猪丹毒(G4T10)活疫苗培养菌数,采用加NaOH调pH增菌和加吐温-80增菌两种方法进行比较试验,前一种方法生产152批,平均培养菌数为7.7×109CFU/mL。后一种方法生产50批,平均菌数为1.792×1010CFU/mL。结果表明,后一种方法较为理想。In order to increase the breeding quantity of the swine erysipelas(G4T10) live vaccine, two (comparing tests) were done. One was to adjust pH with alkali, and the other was to add Tween-80. 152 batches voccine (produced) with the former method had average bacteria of 7.7×10~9 CFU/mL. 50 batches vaccine produced with the latter method had the average bacteria of 1.792×10^(10) CFU/mL. The results showed that the latter is better.

关 键 词：猪丹毒 培养基 培养菌数 活疫苗 NAOH PH 吐温-80 

分 类 号：S858.285.1[农业科学—临床兽医学]