分泌性中耳炎豚鼠的咽鼓管表面活性物质变化  

Change of eustachian tube surfactant of guinea pigs suffering from secretory otitis media

作　　者：廖志苏[1] 陈超[2] 杜瀚[1] 吴秀玲[3] 朱金昌[3] 李智渊[1] 

机构地区：[1]温州医学院附属第一医院耳鼻咽喉科,浙江温州325000 [2]复旦大学儿科医院 [3]温州医学院附属第一医院病理科,浙江温州325000

出　　处：《中华耳鼻咽喉科杂志》2003年第2期101-103,共3页Chinese Journal of Otorhinolaryngology

基　　金：浙江省教育委员会科研项目 (19990 43 3 )

摘　　要：目的　观察豚鼠分泌性中耳炎时咽鼓管内表面活性物质的变化 ,探讨表面活性物质在分泌性中耳炎发病中的作用。方法　建立豚鼠分泌性中耳炎动物模型 ,分析咽鼓管内表面活性物质的生化成分和活性变化 ,同时观察给外源性表面活性物质治疗后咽鼓管内表面活性物质的变化。结果　豚鼠分泌性中耳炎模型组表面活性物质主要成分减少 ,磷脂酰胆碱 (phosphatidylcholine ,PC)和磷脂酰乙醇胺 (phosphatidylethanolamine ,PE)分别是 (19 9± 1 7) %和 (36 8± 2 7) % ,而正常组的PC和PE分别是 (2 5 7± 2 1) %和 (43 7± 3 8) % ,P值均 <0 0 0 1。模型组最低表面张力 (minimumsurfacetension ,γmin)为 (18 5± 2 4 )mN/m ,比正常组 (7 6± 0 8)mN/m升高 ,P <0 0 0l。给外源性表面活性物质治疗后 ,PC和PE增高 ,分别为 (2 3 3± 2 2 ) %和 (42 5± 3 6 ) % ,γmin降低 ,为 (11 8± 2 3)mN/m。结论　豚鼠分泌性中耳炎时咽鼓管内表面活性物质的主要生化成分明显减少 ,活性减弱 。Objective To observe the change of surface active agents of eustachian tube of guinea pigs suffering from secretory otitis media,to explore the effects of surface active agents on secretory otitis media Methods To establish the animal model of guinea pigs suffering from secretory otitis media.To analyse the changes of biochemical component and activity of eustachian tube ′s surfactant,meanwhile;to observe surfactant change after ectogenic surfactant treatment.Results The main components of the surfactant decrease in the model group of guinea pigs suffering from secretory otitismedia,phosphatidylcholine(PC) is (19 9±1 7)%, phosphatidylethanolamine(PE) is (36 8±2 7)%, minimum surface tension (γmin) is (18 5 ± 2 4)mN/m, and PC is (25 7±2 1)%, PE is (43 7±3 8)%, ( P <0 001), and γmin is (7 6±0 8)mN/m in normal group ( P <0 001) After giving Ectogenic surfactant treatment, PC and PE increase, PC is (23 3 ±2 2)%, PE is (42 5 ±3 6)%; γmin reduce,it is (11 8±2 3)mN/m Conclusions The main biochemical componens of the surfactant obviously decrease,the activity of the surfactant weakens in secretory otitis media of guinea pig This study showed the treatment of Ectognic surfactant is effective

关 键 词：分泌性中耳炎 豚鼠 咽鼓管 表面活性物质 发病机制 磷脂复合物 

分 类 号：R764.21[医药卫生—耳鼻咽喉科]