应用放射性微球技术测定兔骨和肌肉组织中血流量  

Radioactive microsphere techniques in quantitative determination of bone and muscle blood flow in rabbits

作　　者：康庆林[1] 张春才[1] 高堂成[1] 孔令山[2] 

机构地区：[1]第二军医大学长海医院骨科,上海200433 [2]长海医院核医学科

出　　处：《第二军医大学学报》2004年第2期189-191,共3页Academic Journal of Second Military Medical University

基　　金：第二军医大学长海医院"2 5 8"基金( 2 0 0 0 2 5 86)

摘　　要：目的 :应用放射性微球技术定量检测实验兔不同骨骼和肌肉组织的血流量。 方法 :以蟾蜍红细胞经 99m Tc标记后制成放射性微球 (1× 1 0 6 / m l) ,将微球注入 1 0只新西兰兔体内 ,检测并计算其四肢长骨干、肱二头肌和股四头肌以及肱骨不同部位组织的血流量。 结果 :以四肢长骨干血流量最大 ,股骨血流量为 (8.94± 0 .4 5 ) m l/ (min· 1 0 0 g) ;肱骨干骺端血流量为(7.73± 0 .6 8) m l/ (min· 1 0 0 g) ,明显高于骨干和骨骺部 (P<0 .0 1 )。肱二头肌血流量为 (1 4 .5 4± 0 .4 5 ) ml/ (min· 1 0 0 g) ,明显高于股四头肌 (P<0 .0 1 )。 结论 :应用放射性微球技术测量动物骨和肌肉组织血流量 。Objective:To quantitatively measure the blood flow of bone and muscle tissue by the radioactive microsphere techniques.Methods:The erythrocytes of hoptoad labelled with the 99m Tc were used as the radioactive microsperes.Each injection consisted of 10 6 microspheres.The blood flow of bone and muscle tissue of 10 rabbits were examined via injection of hoptoad erythrocytes labelled with the isotope; the blood flow of tissues were calculated according to the artery withdraw rate and radioactive counting of samples and reference blood.Results:The blood flow of femurs [(8.94±0.45) ml/(min·100 g)] was the highest among all the long bones,the blood flow of humeral metaphysis [(7.73±0.68) ml/(min·100 g)] was significantly higher than that of diaphysis and epiphysis ( P <0.01),and the flow of musculus biceps brachii [(14.54±0.45) ml/ (min·100 g)] was higher than that of musculus quadriceps femoris ( P <0.01).Conclusion:The hoptoad erythrocytes are suitable to be used as radioactive microspheres.The radioactive microspheres technique is an accurate and practical method for measuring the blood flow of bone and muscle.

关 键 词：放射性微球技术 测定 兔 肌肉组织 血流量 骨骼组织 生物学固定 骨折 

分 类 号：R683[医药卫生—骨科学]