辅酶Q_(10)脂质体的质量评价  

Quality evaluation of Coenzyme Q_(10) liposomes

作　　者：张仰眉[1] 陈建明[1] 高申[1] 王巍[1] 费红莉[1] 

机构地区：[1]第二军医大学药学院药剂学教研室,上海200433

出　　处：《第二军医大学学报》2004年第2期218-219,共2页Academic Journal of Second Military Medical University

摘　　要：目的 :建立自制辅酶 Q1 0 脂质体的质量标准。 方法 :采用负染法和 TSM超细颗粒粒度仪 ,观察脂质体的形态和粒径 ;采用反相高效液相色谱 (RP- HPL C)法测定辅酶 Q1 0 的含量 ;采用超滤器测定脂质体的包封率。 结果 :辅酶 Q1 0 脂质体粒径大小均匀 ,平均粒径 0 .1 84 μm;RP- HPL C法测定 ,在 1 .0～ 1 6 .0 μg· ml- 1浓度范围内线性关系良好 ,平均回收率 (1 0 0 .5±0 .6 5 ) %,最低检测限 1 ng;脂质体的包封率达 95 %以上。 结论 :本评价方法重现性好 。Objective: To establish the quality standard for coenzyme Q 10 liposomes. Methods: Negative staining and TSM were used to study the structure and size of liposomes. A RP HPLC method was developed to determine the drug content. The entrapment efficiency was determined with centrifugal ultrafilter. Results: The liposomes were homogeneous and the average size was 0.184 μm. The standard curve was linear over the range of 1.0 16.0 μg/ml for coenzyme Q 10 , the mean recovery was 100.5%, the detection limit was 1 ng, and the entrapment efficiency was above 95%. Conclusion: The method is convenient, simple and practical for evaluating the quality of coenzyme Q 10 liposomes.

关 键 词：辅酶Q10 脂质体 质量评价 含量测定 包封率 

分 类 号：R977.3[医药卫生—药品] R927.11[医药卫生—药学]