血管紧张素Ⅱ和血管紧张素1-7对内皮细胞释放组织纤溶酶原激活物及其抑制剂-1的影响  

The effects of angiotensinⅡand angiotensin-(1-7) on thesecretion of t-PA and PAI-1 in endothelial cells

作　　者：陈弹[1] 程绪杰[1] 李红霞[1] 杨向军[1] 刘志华[1] 蒋文平[1] 

机构地区：[1]苏州大学附属第一医院心内科,江苏苏州215006

出　　处：《临床心血管病杂志》2004年第2期99-101,共3页Journal of Clinical Cardiology

摘　　要：目的 :研究血管紧张素Ⅱ (AngⅡ )和血管紧张素 1 7[Ang ( 1 7) ]对内皮细胞分泌组织纤溶酶原激活物 (t PA)和纤溶酶原激活物抑制剂 1(PAI 1)的影响。方法 :培养内皮细胞株 (ECV30 4 ) ,分为AngⅡ和Ang ( 1 7)刺激组 ,培养基中AngⅡ和Ang ( 1 7)加至终浓度为 5、10、2 5、5 0、10 0nmol/L ,2 4h后测定上清液中的t PA和PAI 1的含量。结果 :内皮细胞在AngⅡ终浓度为 5 0、10 0nmol/L组刺激 2 4h后 ,其分泌的PAI 1含量较对照组有明显升高 ,差异有统计学意义 (P <0 .0 5 )。而Ang ( 1 7)在同样浓度组却显著降低PAI 1(P <0 .0 5 ) ,AngⅡ和Ang ( 1 7)两组t PA含量差异无统计学意义 ( P >0 .0 5 )。结论 :AngⅡ对内皮细胞株分泌的PAI 1有显著的升高作用 ,而Ang ( 1 7)对PAI 1作用相反。两者对t PA均无显著影响。提示AngⅡ和Ang ( 1 7)Objective:To investigate the effects of angiotensinⅡ(AngⅡ)and angiotensin (1 7)[Ang (1 7)] on the secretion of t PA and PAI 1 in eddothetial cells..Methods:Cultivated ECV 304 cells were stimulated with AngⅡ(5,10,25,50,100 nmol/L)and Ang (1 7)(5,10,25,50,100 nmol/L) for 24 hours. Tissue type plasminogen activator (t PA) and plasminogen activator inhibitor 1(PAI 1) in the supernatant of the cell culture were measured by enzyme linked immunosorbent assay(ELISA).Results:The concentrations of t PA had no statistically significant changes whether the ECV304 cells were stimulated by AngⅡor Ang (1 7) .But PAI 1 was significantly increased by AngⅡ(50、100nmol/L)and decreased by Ang (1 7) at the same concentration.Conclusion:AngⅡand Ang (1 7) has no effects on t PA released from ECV304.But, PAI 1 can be increased by AngⅡ and decreased by Ang (1 7) . AngⅡor Ang (1 7) may play a important role in the regulation of fibrinolysis.

关 键 词：血管紧张素Ⅱ 血管紧张素1-7 内皮血管 组织纤溶酶原激活物 纤溶酶原激活物抑制剂-1 

分 类 号：R972[医药卫生—药品] Q514.3[医药卫生—药学]