RP-HPLC法测定阿莫西林—盐酸环丙沙星可溶性粉的含量  

Determination of amoxicillin and ciprofloxacin hydrochloride inamoxicillin & ciprofloxacin hydrochloride soluble powder by RP-HPLC

作　　者：张小华[1] 张龙[1] 李生其[1] 邢晓玲[1] 朱丽霞[1] 冯艺[1] 

机构地区：[1]江苏畜牧兽医职业技术学院药物检测中心,江苏泰州225300

出　　处：《中国兽药杂志》2004年第2期26-28,共3页Chinese Journal of Veterinary Drug

摘　　要：　应用RP-HPLC法测定阿莫西林—盐酸环丙沙星可溶性粉的含量。采用Shim-packCLC-ODSC18柱(150mm×6mm,5μm),以1.5×10-2mol/L三乙胺溶液(用磷酸调pH至3.0)-乙腈(体积比85∶15)为流动相,流速为1.0mL/min,检测波长为229nm,柱温为40℃。在此色谱条件下两者能完全分离,阿莫西林、盐酸环丙沙星分别在22～108μg/mL和29～146μg/mL范围内峰面积与浓度呈良好的线性关系,相关系数均为0.9999。阿莫西林、盐酸环丙沙星的回收率分别为99.50%～100.20%和99.30%～99.86%(n=5);RSD分别为0.60%～1.00%和0.86%～1.02%(n=5)。本法可用于阿莫西林—盐酸环丙沙星可溶性粉的质量控制。A RP-HPLC method for determination of the content of amoxicillin and ciprofloxacin hydrochloride in the soluble powder was established. Shim-pack CLC-ODS column (150 mm×6 mm, 5 μm) was used with 1.5×10^(-2)mol/L (triethylamine) (it's pH was adjusted to 3.0 with phosphoric acid )-acetonitrile (85∶15,(V/V)) as the mobile phase at flow rate of (1.0 mL/min.) The detection wavelength was 229 nm. The temperature of column was coutroled at 40 ℃. Under the (chromatographic) conditions, the amoxicillin and ciprofloxacin hydrochloride could be completely (separated.) The standard curves for amoxicillin and ciprofloxacin hydrochloride were linear in the concentration range of 22～108 μg/mL and 29～146 μg/mL (r=0.999 9,n=5), respectively. The recoveries of (amoxicillin) and ciprofloxacin hydrochloride were 99.50% ～ 100.20% (RSD = 0.60% ～ 1.00%) and 99.30% ～ 99.86% (RSD=0.86% ～ 1.02%),n=5, respectively. The method could be used to control the quality of amoxicillin & ciprofloxacin hydrochloride soluble (powder.)

关 键 词：RP-HPLC法 阿莫西林 盐酸环丙沙星 可溶性粉 质量控制 

分 类 号：S859.796[农业科学—临床兽医学] S859.799.1[农业科学—兽医学]