RP-HPLC法同时测定人血清中阿莫西林、克拉维酸钾浓度  

Determination of Amoxycillin & Clavulanate Potassium in Human Serum by RP-HPLC

作　　者：王川平[1] 任进民[1] 孙倩[1] 张晓青[2] 徐智儒[2] 

机构地区：[1]河北医科大学第二医院药剂科,石家庄050000 [2]河北医科大学药学院

出　　处：《中国药师》2004年第3期182-184,共3页China Pharmacist

摘　　要：目的 :建立RP HPLC法同时测定人血清中阿莫西林、克拉维酸钾的浓度。方法 :采用LunaC18色谱柱 (2 5 0mm× 4 .6mm ,5 μm) ,以 0 .0 5M磷酸盐缓冲液 乙腈 (90∶10 ;磷酸调pH至 2 .30 )为流动相 ,流速 1ml·min-1。检测波长为 2 2 0nm。结果 :CLV在 0 .0 6 6～ 16 .86 μg·ml-1,范围内有良好线性关系 ,r=0 .9999(n =9) ;AMO在 0 .12～ 2 9.4 5 μg·ml-1,范围内有良好线性关系r=0 .9998(n =9)。本法测定血清中CLV最低检测浓度为 0 .0 6 0 μg·ml-1,AMO为 0 .10 μg·ml-1。 (S/N≥ 3)。平均方法回收率为 97.5 %。结论 :本法简便 ,准确 ,重现性好 ,用于阿莫西林克拉维酸钾分散片人血清中血药浓度测定取得良好结果。Objective: A simple RP-HPLC method was established for the determination of AMO & CLV in human serum.Method: The chromatographic separation was carried out on Luna C 18 column(250 mm× 4.6 mm, 5 μm) with a mobile phase consisting of 0.05M NaH 2PO 4 buffer-acetonitrile (90∶10, adjust pH to 2.3 with H 3PO 4), flow rate was 1 ml·min -1. The detecting wavelength was at 220 nm.Result: The linear range of CLV and AMO were 0.066- 16.86 μg·ml -1, 0.12- 29.45 μg·ml -1,respectively. The minimum detection limit of CLV and AMO were 0.060 μg·ml -1, 0.10 μg·ml -1(S/N=3),respectively;The average recover in two drugs rates were 97.5%. Conclusion: This method is simple, accurate, and reproducible. It has been applied successfully to the determination of AMO and CLV dispersible tablets in human serum for pharmacokinetic studies.

关 键 词：阿莫西林 克拉维酸钾 反相高效液相色谱法 血药浓度 

分 类 号：R927.2[医药卫生—药学] R978.11